Vanacker J M, Bonnelye E, Delmarre C, Laudet V
CNRS UMR 49, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, France.
Oncogene. 1998 Nov 12;17(19):2429-35. doi: 10.1038/sj.onc.1202167.
The superfamily of nuclear receptors comprises transcription factors that depend on a ligand for their activity. In addition, the superfamily includes a number of orphan receptors, for which no ligand is known. We report here that the orphan receptor estrogen receptor related alpha receptor (ERR alpha) stimulates the expression of the thyroid hormone receptor alpha (TR alpha) gene promoter. We characterized a responsive site that is both necessary and sufficient for ERR alpha-induced transactivation. In addition, we show that both TR alpha and ERR alpha are coexpressed in embryonic intestine, brown fat and heart as well as in the adult gonads. In the testis, expression of both receptors can be found in the seminiferous tubes where it is totally restricted to spermatocytes I. Altogether this suggests that TR alpha is an in vivo target of ERR alpha.
核受体超家族由依赖配体发挥活性的转录因子组成。此外，该超家族还包括一些尚未发现配体的孤儿受体。我们在此报告，孤儿受体雌激素受体相关α受体（ERRα）可刺激甲状腺激素受体α（TRα）基因启动子的表达。我们鉴定出一个对ERRα诱导的反式激活既必要又充分的反应位点。此外，我们发现TRα和ERRα在胚胎期的肠道、棕色脂肪和心脏以及成年性腺中共同表达。在睾丸中，两种受体的表达均可在生精小管中检测到，且完全局限于初级精母细胞。这一切表明TRα是ERRα在体内的靶标。
