Murray G I, Duncan M E, Arbuckle E, Melvin W T, Fothergill J E
Department of Pathology, University of Aberdeen, UK.
Gut. 1998 Dec;43(6):791-7. doi: 10.1136/gut.43.6.791.
The matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) are strongly implicated in tumour invasion and metastasis.
To investigate the presence of individual MMPs and TIMPs in gastric cancer.
The presence of MMP-1, MMP-2, MMP-3, MMP-9, TIMP-1, and TIMP-2 was identified in a group of gastric cancers (n=74) by immunohistochemistry using monoclonal antibodies. These antibodies were effective on formalin fixed, paraffin wax embedded sections.
A large proportion (94%) of gastric cancers contained MMP-2; MMP-1 and MMP-9 were also detected in 73% and 70% of tumours respectively. MMP-3 was only present in 27% of tumours. MMP-1 and MMP-9 were found predominantly in intestinal type tumours. TIMP-1 and TIMP-2 were identified in 41% and 57% of tumours respectively. Immunoreactivity for individual MMPs or TIMPs was not identified in normal stomach.
This study shows the presence of matrix metalloproteinases, particularly MMP-2, and TIMPs in stomach cancer. Antibodies which are effective in formalin fixed, paraffin wax embedded sections are useful for the identification of MMPs and TIMPs in diagnostic specimens.
基质金属蛋白酶（MMPs）和基质金属蛋白酶组织抑制剂（TIMPs）与肿瘤侵袭和转移密切相关。
研究胃癌中单个MMPs和TIMPs的存在情况。
使用单克隆抗体通过免疫组织化学在一组胃癌（n = 74）中鉴定MMP - 1、MMP - 2、MMP - 3、MMP - 9、TIMP - 1和TIMP - 2的存在。这些抗体对福尔马林固定、石蜡包埋切片有效。
很大比例（94%）的胃癌含有MMP - 2；分别在73%和70%的肿瘤中也检测到MMP - 1和MMP - 9。MMP - 3仅存在于27%的肿瘤中。MMP - 1和MMP - 9主要在肠型肿瘤中发现。分别在41%和57%的肿瘤中鉴定出TIMP - 1和TIMP - 2。在正常胃中未发现单个MMPs或TIMPs的免疫反应性。
本研究表明胃癌中存在基质金属蛋白酶，尤其是MMP - 2，以及TIMPs。对福尔马林固定、石蜡包埋切片有效的抗体可用于在诊断标本中鉴定MMPs和TIMPs。
