Cann I K, Komori K, Toh H, Kanai S, Ishino Y
Department of Molecular Biology, Biomolecular Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565, Japan.
Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14250-5. doi: 10.1073/pnas.95.24.14250.
We describe here a DNA polymerase family highly conserved in Euryarchaeota, a subdomain of Archaea. The DNA polymerase is composed of two proteins, DP1 and DP2. Sequence analysis showed that considerable similarity exists between DP1 and the second subunit of eukaryotic DNA polymerase delta, a protein essential for the propagation of Eukarya, and that DP2 has conserved motifs found in proteins with nucleotide-polymerizing activity. These results, together with our previous biochemical analyses of one of the members, DNA polymerase II (DP1 + DP2) from Pyrococcus furiosus, implicate the DNA polymerases of this family in the DNA replication process of Euryarchaeota. The discovery of this DNA-polymerase family, aside from providing an opportunity to enhance our knowledge of the evolution of DNA polymerases, is a significant step toward the complete understanding of DNA replication across the three domains of life.
我们在此描述一个在古菌域的一个亚域广古菌门中高度保守的DNA聚合酶家族。该DNA聚合酶由两种蛋白质DP1和DP2组成。序列分析表明，DP1与真核生物DNA聚合酶δ的第二个亚基（一种对真核生物繁殖至关重要的蛋白质）之间存在相当大的相似性，并且DP2具有在具有核苷酸聚合活性的蛋白质中发现的保守基序。这些结果，连同我们之前对其中一个成员——来自嗜热栖热菌的DNA聚合酶II（DP1 + DP2）的生化分析，表明该家族的DNA聚合酶参与广古菌门的DNA复制过程。这个DNA聚合酶家族的发现，除了为增进我们对DNA聚合酶进化的了解提供了一个机会外，也是朝着全面理解生命三个域中的DNA复制迈出的重要一步。
