Saxby L, Rosen E, Boulton M
University Department of Ophthalmology, Manchester Royal Eye Hospital.
Br J Ophthalmol. 1998 Aug;82(8):945-52. doi: 10.1136/bjo.82.8.945.
To study the behaviour of residual lens epithelial cells after capsulorhexis and expression of material from the isolated lens.
Human and bovine lens capsules were isolated, sterile non-toxic silicone rings inserted, and the preparations placed in organ culture for up to 12 weeks. Cell coverage of the posterior lens capsule was recorded and the capsules were examined, both pre- and post-coverage, for (a) proliferative activity and (b) cytoskeletal components.
After a lag period outgrowth was observed across the posterior capsule. The rate of cell coverage was dependent upon both species and the presence or absence of serum. The proliferative activity of the cells was greatest at or near the leading edge and decreased once covered. Wrinkles became visible in the posterior capsule during the late stages of precoverage and increased in both number and complexity. All cells on both the human and bovine posterior capsules contained F-actin and vimentin and the majority were immunolabelled for alpha-smooth muscle actin (alpha-SMA).
This model exhibits many of the in vivo characteristics of the lens capsule after extracapsular surgery and may prove useful in further elucidating the cellular mechanisms of posterior capsule opacification.
研究撕囊后晶状体残留上皮细胞的行为以及分离晶状体物质的表达。
分离人及牛的晶状体囊，插入无菌无毒硅胶环，将制剂置于器官培养中长达12周。记录晶状体后囊的细胞覆盖情况，并在覆盖前后检查囊，以检测（a）增殖活性和（b）细胞骨架成分。
经过一段延迟期后，在后囊上观察到细胞生长。细胞覆盖速率取决于物种以及血清的有无。细胞的增殖活性在前缘或其附近最大，一旦被覆盖则降低。在覆盖前期的后期，后囊中可见皱纹，其数量和复杂性均增加。人及牛后囊上的所有细胞均含有F-肌动蛋白和波形蛋白，且大多数细胞对α-平滑肌肌动蛋白（α-SMA）呈免疫标记阳性。
该模型展现了囊外手术后晶状体囊的许多体内特征，可能有助于进一步阐明后囊混浊的细胞机制。
