泉古菌门小亚基核糖体RNA基因序列的定量测定
Quantitative measure of small-subunit rRNA gene sequences of the kingdom korarchaeota.
作者信息
Brunk CF, Eis N
机构信息
Lehrstuhl fur Mikrobiologie und Archaeenzentrum der Universitat Regensburg, Regensburg, Germany.
出版信息
Appl Environ Microbiol. 1998 Dec;64(12):5064-6. doi: 10.1128/AEM.64.12.5064-5066.1998.
Abstract
Comparative PCR amplification of small-subunit (SSU) rRNA gene (rDNA) sequences indicates substantial preferential PCR amplification of pJP27 sequences with korarchaeote-specific PCR primers. The coamplification of a modified SSU rDNA sequence can be used as an internal standard to determine the amount of a specific SSU rDNA sequence.
摘要
小亚基（SSU）核糖体RNA基因（rDNA）序列的比较性聚合酶链反应（PCR）扩增表明，使用泉古菌特异性PCR引物时，pJP27序列存在显著的优先PCR扩增。修饰的SSU rDNA序列的共扩增可用作内标，以确定特定SSU rDNA序列的量。
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