Verma A, Kanwar K C
Department of Biophysics, Panjab University, Chandigarh, India.
Andrologia. 1998 Nov;30(6):325-9. doi: 10.1111/j.1439-0272.1998.tb01178.x.
Human spermatozoal motility, viability and lipid peroxidation (LPO) have been assessed in Ringer-Tyrode supplemented with different concentrations of ascorbic acid (AA) ranging from 50 to 4000 microM. Ascorbic acid in concentrations below 1000 microM protects spermatozoa from free radical damage as evidenced from improvement in their motility and viability. Concomitantly, there is also witnessed depletion of malondialdehyde generation (an end product of LPO) following AA treatment. Ascorbic acid at 1000 microM concentration and above, however, is not protective, as evidenced by abrupt fall in sperm motility and viability and concomitant increase in LPO.
在补充了浓度范围为50至4000微摩尔不同浓度抗坏血酸（AA）的林格 - 泰罗德溶液中，对人类精子的活力、存活率和脂质过氧化（LPO）进行了评估。浓度低于1000微摩尔的抗坏血酸可保护精子免受自由基损伤，这从其活力和存活率的改善中得到证明。同时，抗坏血酸处理后还可见丙二醛生成（LPO的终产物）减少。然而，浓度为1000微摩尔及以上的抗坏血酸并无保护作用，这从精子活力和存活率的突然下降以及LPO的相应增加中得到证明。
