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花生四烯酸的脂氧合酶代谢产物对人单核骨髓细胞和骨髓基质细胞培养生长的影响。

Effects of lipoxygenase metabolites of arachidonic acid on the growth of human mononuclear marrow cells and marrow stromal cell cultures.

作者信息

Desplat V, Dupuis F, Trimoreau F, Dulery C, Praloran V, Denizot Y

机构信息

Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France.

出版信息

Mediators Inflamm. 1998;7(1):31-3. doi: 10.1080/09629359891351.

DOI:10.1080/09629359891351
PMID:9839696
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1781818/
Abstract

The effects of various lipoxygenase metabolites of arachidonic acid (AA) were investigated on the growth of freshly isolated human bone marrow mononuclear cells and marrow stromal cell cultures. LTB4, LXA4, LXB4, 12-HETE and 15-HETE (1 microM) decreased [3H]-thymidine incorporation on marrow stromal cell cultures without affecting cell number. Only 12-HETE showed a dose-response effect on [3H]-thymidine incorporation. While LTB4 (1 microM) decreased thymidine incorporation on marrow mononuclear cells, LTC4, LXA4, LXB4, 12-HETE and 15-HETE had no effect. The lipoxygenase inhibitor NDGA had no effect on both cell types suggesting no role of endogenous lipoxygenase metabolites on cell growth. These results suggest no important role of lipoxygenase metabolites of AA on the proliferation of human marrow mononuclear cells and marrow stromal cell cultures.

摘要

研究了花生四烯酸（AA）的各种脂氧合酶代谢产物对新鲜分离的人骨髓单个核细胞生长及骨髓基质细胞培养的影响。白三烯B4（LTB4）、白三烯A4（LXA4）、白三烯B4（LXB4）、12-羟基二十碳四烯酸（12-HETE）和15-羟基二十碳四烯酸（15-HETE）（1微摩尔）可降低骨髓基质细胞培养中[3H] - 胸腺嘧啶核苷的掺入，而不影响细胞数量。只有12-HETE对[3H] - 胸腺嘧啶核苷掺入呈现剂量反应效应。虽然LTB4（1微摩尔）可降低骨髓单个核细胞中胸腺嘧啶核苷的掺入，但白三烯C4（LTC4）、LXA4、LXB4、12-HETE和15-HETE则无此作用。脂氧合酶抑制剂去甲二氢愈创木酸（NDGA）对这两种细胞类型均无影响，提示内源性脂氧合酶代谢产物对细胞生长无作用。这些结果表明，AA的脂氧合酶代谢产物对人骨髓单个核细胞和骨髓基质细胞培养的增殖无重要作用。

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