Richt J A, Clements J E, Herzog S, Pyper J, Wahn K, Becht H
Institut für Virologie, Justus-Liebig-Universität Giessen, Germany.
Med Microbiol Immunol. 1993 Nov;182(5):271-80. doi: 10.1007/BF00579625.
Treatment of homogenates from Borna disease virus (BDV)-infected brain tissue or cell cultures with Freon-113 yielded infectious particles with a buoyant density of 1.16-1.22 g/ml. Positive- and negative-stranded BDV-specific RNA species as well as three virus-specific proteins, known to be present in BDV-infected cell extracts, were demonstrated in these Freon-treated fractions. When the Freon-purified virus preparations were treated with RNase A prior to RNA extraction, only negative-stranded, genomic RNA was detected in Northern blot hybridizations using sense and antisense RNA probes. These data substantiate that BDV is a negative-stranded RNA virus.
用氟利昂-113处理博尔纳病病毒(BDV)感染的脑组织或细胞培养物的匀浆,可产生浮力密度为1.16 - 1.22 g/ml的感染性颗粒。在这些经氟利昂处理的组分中,证实存在正链和负链BDV特异性RNA种类以及三种已知存在于BDV感染细胞提取物中的病毒特异性蛋白。当在RNA提取之前用核糖核酸酶A处理经氟利昂纯化的病毒制剂时,在使用正义和反义RNA探针的Northern印迹杂交中仅检测到负链基因组RNA。这些数据证实BDV是一种负链RNA病毒。