通过16S rRNA基因PCR快速鉴定临床相关诺卡氏菌属菌种至属水平
Rapid identification of clinically relevant Nocardia species to genus level by 16S rRNA gene PCR.
作者信息
Laurent F J, Provost F, Boiron P
机构信息
Institut Pasteur, Unite de Mycologie, Centre National de Reference des Mycoses Humaines, des Antifongiques et des Actinomycetes, 75724 Paris Cedex 15, France.
出版信息
J Clin Microbiol. 1999 Jan;37(1):99-102. doi: 10.1128/JCM.37.1.99-102.1999.
Abstract
Two regions of the gene coding for 16S rRNA in Nocardia species were selected as genus-specific primer sequences for a PCR assay. The PCR protocol was tested with 60 strains of clinically relevant Nocardia isolates and type strains. It gave positive results for all strains tested. Conversely, the PCR assay was negative for all tested species belonging to the most closely related genera, including Dietzia, Gordona, Mycobacterium, Rhodococcus, Streptomyces, and Tsukamurella. Besides, unlike the latter group of isolates, all Nocardia strains exhibited one MlnI recognition site but no SacI restriction site. This assay offers a specific and rapid alternative to chemotaxonomic methods for the identification of Nocardia spp. isolated from pathogenic samples.
摘要
在诺卡氏菌属物种中，编码16S rRNA的基因的两个区域被选作PCR检测的属特异性引物序列。该PCR方案用60株临床相关的诺卡氏菌分离株和模式菌株进行了测试。对所有测试菌株均得到阳性结果。相反，对于所有属于最密切相关属的测试物种，包括迪茨氏菌属、戈登氏菌属、分枝杆菌属、红球菌属、链霉菌属和冢村氏菌属，PCR检测均为阴性。此外，与后一组分离株不同，所有诺卡氏菌菌株都有一个MlnI识别位点，但没有SacI限制位点。该检测方法为从致病样本中分离出的诺卡氏菌属物种的鉴定提供了一种比化学分类方法更特异、快速的替代方法。
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