产甲烷古菌中的（R）-柠苹酸合酶
(R)-citramalate synthase in methanogenic archaea.
作者信息
Howell D M, Xu H, White R H
机构信息
Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0308, USA.
出版信息
J Bacteriol. 1999 Jan;181(1):331-3. doi: 10.1128/JB.181.1.331-333.1999.
Abstract
The Methanococcus jannaschii gene MJ1392 was cloned, and its protein product was hyperexpressed in Escherichia coli. The resulting protein was purified and shown to catalyze the condensation of pyruvate and acetyl coenzyme A, with the formation of (R)-citramalate. Thus, this gene (cimA) encodes an (R)-citramalate synthase (CimA). This is the first identification of this enzyme, which is likely involved in the biosynthesis of isoleucine.
摘要
詹氏甲烷球菌基因MJ1392被克隆出来，其蛋白质产物在大肠杆菌中得到了高效表达。所得蛋白质经过纯化后，被证明能够催化丙酮酸和乙酰辅酶A的缩合反应，生成（R）-柠苹酸。因此，该基因（cimA）编码一种（R）-柠苹酸合酶（CimA）。这是对这种酶的首次鉴定，它可能参与异亮氨酸的生物合成。
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