酿酒酵母线粒体基因组的完整序列。

The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae.

作者信息

Foury F, Roganti T, Lecrenier N, Purnelle B

机构信息

Unité de Biochimie Physiologique, Louvain-la-Neuve, Belgium.

出版信息

FEBS Lett. 1998 Dec 4;440(3):325-31. doi: 10.1016/s0014-5793(98)01467-7.

DOI:10.1016/s0014-5793(98)01467-7

PMID:9872396

Abstract

The currently available yeast mitochondrial DNA (mtDNA) sequence is incomplete, contains many errors and is derived from several polymorphic strains. Here, we report that the mtDNA sequence of the strain used for nuclear genome sequencing assembles into a circular map of 85,779 bp which includes 10 kb of new sequence. We give a list of seven small hypothetical open reading frames (ORFs). Hot spots of point mutations are found in exons near the insertion sites of optional mobile group I intron-related sequences. Our data suggest that shuffling of mobile elements plays an important role in the remodelling of the yeast mitochondrial genome.

摘要

目前可用的酵母线粒体DNA（mtDNA）序列不完整，包含许多错误，并且来自多个多态性菌株。在此，我们报告用于核基因组测序的菌株的mtDNA序列组装成一个85,779 bp的环状图谱，其中包括10 kb的新序列。我们列出了七个小的假定开放阅读框（ORF）。在可选的I类移动内含子相关序列插入位点附近的外显子中发现了点突变热点。我们的数据表明，移动元件的重排对酵母线粒体基因组的重塑起着重要作用。