Elekes K, Nässel D R
Department of Experimental Zoology, Balaton Limnological Research Institute of the Hungarian Academy of Sciences, Fürdötelepi u. 3., P.O. Box 35, H-8237 Tihany, Hungary.
Cell Tissue Res. 1999 Feb;295(2):339-48. doi: 10.1007/s004410051240.
By using an antiserum raised against a crustacean beta-pigment-dispersing hormone (PDH), the distribution and chemical neuroanatomy of PDH-like immunoreactive neurons was investigated in the central nervous system of the gastropod snails, Helix pomatia and Lymnaea stagnalis. The number of immunoreactive cells in the Helix central nervous system was found to be large (700-900), whereas in Lymnaea, only a limited number (50-60) of neurons showed immunoreactivity. The immunostained neurons in Helix were characterized by rich arborizations in all central ganglia and revealed massive innervation of all peripheral nerves and the neural (connective tissue) sheath around the ganglia and peripheral nerve trunks. A small number of Helix nerve cell bodies in the viscero-parietal ganglion complex were also found to be innervated by PDH-like immunoreactive processes. Hence, a complex central and peripheral regulatory role, including neurohormonal actions, is suggested for a PDH-like substance in Helix, whereas the sites of action may be more limited in Lymnaea.
通过使用针对一种甲壳类β-色素分散激素（PDH）产生的抗血清，在腹足纲蜗牛（罗马蜗牛和椎实螺）的中枢神经系统中研究了类PDH免疫反应性神经元的分布和化学神经解剖学。发现罗马蜗牛中枢神经系统中的免疫反应性细胞数量众多（700 - 900个），而在椎实螺中，只有有限数量（50 - 60个）的神经元显示出免疫反应性。罗马蜗牛中免疫染色的神经元的特征是在所有中枢神经节中有丰富的分支，并显示出对所有外周神经以及神经节和外周神经干周围的神经（结缔组织）鞘有大量的神经支配。在内脏 - 顶叶神经节复合体中的少数罗马蜗牛神经细胞体也被发现受到类PDH免疫反应性突起的支配。因此，对于罗马蜗牛中的一种类PDH物质，提示其具有包括神经激素作用在内的复杂的中枢和外周调节作用，而在椎实螺中其作用位点可能更有限。
