Kawamura Y
J Natl Cancer Inst. 1976 May;56(5):927-30. doi: 10.1093/jnci/56.5.927.
The correlation of murine leukemia virus (MuLV) infection and leukemogenesis was examined by XC plaque assay in W/F rats neonatally infected with the rat-adapted Gross virus (RAGV). Thymic lymphomas developed in 100% of infected rats, with a mean latent period of 64 days. The virus infectivity was first detected in the thymus as early as 15 days after the virus infection; the titer steadily increased thereafter until the development of thymic lymphoma. No other tissues of virus-infected rats showed virus infectivity until the development of localized thymic lymphoma, though sera of a few rats showed low infectivity titers. In rats with generalized leukemia, however, high titers were detected in the sera and leukemic tissues. In untreated controls, all tissues tested invariably showed negative titers for MuLV infectivity at any age up to 7 months after birth. The results indicated that those tissue sites were common to both RAGV infectivity and the leukemogenic process, with the primary involvement of the thymus, and that the appearance of RAGV infectivity in various tissues represented the expression of the oncogenic genome of RAGV.
通过XC空斑试验，对新生期感染大鼠适应型格罗斯病毒（RAGV）的W/F大鼠进行研究，以检测鼠白血病病毒（MuLV）感染与白血病发生之间的相关性。100%的感染大鼠发生了胸腺淋巴瘤，平均潜伏期为64天。早在病毒感染后15天，就在胸腺中首次检测到病毒感染性；此后病毒滴度稳步上升，直至胸腺淋巴瘤发生。在局限性胸腺淋巴瘤发生之前，病毒感染大鼠的其他组织均未显示出病毒感染性，不过少数大鼠的血清显示出低感染性滴度。然而，在患有全身性白血病的大鼠中，血清和白血病组织中检测到高滴度。在未经处理的对照组中，在出生后7个月内的任何年龄，所有检测组织的MuLV感染性滴度均始终为阴性。结果表明，这些组织部位是RAGV感染性和白血病发生过程所共有的，胸腺是主要受累器官，并且RAGV在各种组织中的感染性出现代表了RAGV致癌基因组的表达。
