Vande Broek A, Lambrecht M, Eggermont K, Vanderleyden J
F. A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, B-3001 Heverlee, Belgium.
J Bacteriol. 1999 Feb;181(4):1338-42. doi: 10.1128/JB.181.4.1338-1342.1999.
Transcription of the Azospirillum brasilense ipdC gene, encoding an indole-3-pyruvate decarboxylase involved in the biosynthesis of indole-3-acetic acid (IAA), is induced by IAA as determined by ipdC-gusA expression studies and Northern analysis. Besides IAA, exogenously added synthetic auxins such as 1-naphthaleneacetic acid, 2,4-dichlorophenoxypropionic acid, and p-chlorophenoxyacetic acid were also found to upregulate ipdC expression. No upregulation was observed with tryptophan, acetic acid, or propionic acid or with the IAA conjugates IAA ethyl ester and IAA-L-phenylalanine, indicating structural specificity is required for ipdC induction. This is the first report describing the induction of a bacterial gene by auxin.
通过ipdC - gusA表达研究和Northern分析确定，编码参与吲哚 - 3 - 乙酸（IAA）生物合成的吲哚 - 3 - 丙酮酸脱羧酶的巴西固氮螺菌ipdC基因的转录受IAA诱导。除IAA外，还发现外源添加的合成生长素如1 - 萘乙酸、2,4 - 二氯苯氧基丙酸和对氯苯氧基乙酸也能上调ipdC表达。用色氨酸、乙酸或丙酸或IAA共轭物IAA乙酯和IAA - L - 苯丙氨酸未观察到上调，表明ipdC诱导需要结构特异性。这是描述生长素诱导细菌基因的首篇报道。
