格拉夫卵泡是L-抗坏血酸积累的部位。
The Graafian follicle is a site of L-ascorbate accumulation.
作者信息
Paszkowski T, Clarke R N
机构信息
Department of Gynecology, University School of Medicine, Lublin, Poland.
出版信息
J Assist Reprod Genet. 1999 Jan;16(1):41-5. doi: 10.1023/a:1022597629622.
PURPOSE
Our purpose was to evaluate the L-ascorbate level in human preovulatory follicular fluid and to quantify the blood/follicle gradient for vitamin C. The effect of smoking on the follicular L-ascorbate concentration was studied. The correlations were tested between follicular L-ascorbate and follicle size and oocyte maturity.
METHODS
In 65 women undergoing in vitro fertilization, samples of follicular fluid and blood serum were collected. Biochemical analyses included L-ascorbate determinations by a colorimetric method and cotinine measurements by a radioimmunoassay.
RESULTS
The average follicular fluid:serum ratio for L-ascorbate was 1:68. Ascorbate levels in follicular fluid and serum were significantly correlated. The follicular L-ascorbate level did not correlate with the follicle size and the oocyte maturity grade. Insignificantly lowered follicular L-ascorbate levels were observed in smokers.
CONCLUSIONS
The extracellular compartment of the Graafian follicle is a site of an ascorbate accumulation. Exposure to tobacco smoke does not significantly diminish the intrafollicular pool of L-ascorbate.
目的
我们的目的是评估人类排卵前卵泡液中的L - 抗坏血酸水平，并量化维生素C的血液/卵泡梯度。研究了吸烟对卵泡L - 抗坏血酸浓度的影响。测试了卵泡L - 抗坏血酸与卵泡大小和卵母细胞成熟度之间的相关性。
方法
在65名接受体外受精的女性中，收集卵泡液和血清样本。生化分析包括用比色法测定L - 抗坏血酸和用放射免疫分析法测定可替宁。
结果
L - 抗坏血酸的平均卵泡液：血清比值为1:68。卵泡液和血清中的抗坏血酸水平显著相关。卵泡L - 抗坏血酸水平与卵泡大小和卵母细胞成熟度等级无关。在吸烟者中观察到卵泡L - 抗坏血酸水平略有降低。
结论
格拉夫卵泡的细胞外区是抗坏血酸积累的部位。接触烟草烟雾不会显著减少卵泡内L - 抗坏血酸的储备。
Zotero 插件