葡萄球菌蛋白A；其制备方法及在风疹血清学中的应用。
Staphylococcal protein A; its preparation and an application to rubella serology.
作者信息
Mallinson H, Roberts C, Bruce White G B
出版信息
J Clin Pathol. 1976 Nov;29(11):999-1002. doi: 10.1136/jcp.29.11.999.
Abstract
Good yields of staphylococcal protein A are obtained by growing the staphylococcus Cowan type 1 on cellophane agar. The activity of these preparations in removing immunoglobulin G (IgG) from human serum can be readily measured by the Mancini radial-diffusion technique and the correct in-use dilution determined. Treatment with protein A of sera from women with a history of rubella may help in the identification of those having specific antibody in the IgM and IgA fractions. This relatively simple procedure may have worthwhile application in the diagnosis of rubella.
摘要
通过在玻璃纸琼脂上培养 Cowan 1 型葡萄球菌可获得高产率的葡萄球菌蛋白 A。这些制剂从人血清中去除免疫球蛋白 G（IgG）的活性可通过 Mancini 径向扩散技术轻松测定，并确定正确的使用稀释度。用蛋白 A 处理有风疹病史女性的血清，可能有助于鉴定 IgM 和 IgA 组分中具有特异性抗体的个体。这个相对简单的程序在风疹诊断中可能有重要应用。
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Infect Immun. 1971 Jan;3(1):10-5. doi: 10.1128/iai.3.1.10-15.1971.
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