Xia S Z, Feng C H, Guo A K
Laboratory of Visual Information Processing, Institute of Biophysics, Academia Sinica, Beijing, PR China.
Physiol Behav. 1999;65(4-5):617-23. doi: 10.1016/s0031-9384(98)00191-7.
Memory consolidation in Drosophila was investigated using cold anesthesia- and hypoxia-induced amnesia. Individual flies were operantly trained to avoid the specific flight orientations with respect to the landmarks surrounding them when paired with heat reinforcement at a flight simulator. Cold anesthesia, introduced immediately after training, exerted a significantly diminishing effect on memory between 15 and 150 min after training. Hypoxia delivered immediately after training had a significantly diminishing effect on memory between 30 and 150 min after training. In addition, cold anesthesia disrupted memory only when introduced within the first 20 min, while hypoxia worked only when delivered within the first 2 min after training. When interpreted in the context of a four-phase model of memory consolidation, the results suggest that 1) cold anesthesia disrupts both short-term memory (STM) and anesthesia-resistant memory (ARM), 2) hypoxia disrupts ARM specifically, 3) both of them leave long-term memory (LTM) intact, and 4) LTM may be independent of availability of STM and ARM in flies.
利用冷麻醉和缺氧诱导的失忆对果蝇的记忆巩固进行了研究。在飞行模拟器中，当个体果蝇与热强化配对时，对其进行操作性训练，使其避免相对于周围地标物的特定飞行方向。训练后立即引入冷麻醉，在训练后15至150分钟内对记忆产生显著的减弱作用。训练后立即给予缺氧处理，在训练后30至150分钟内对记忆有显著的减弱作用。此外，冷麻醉仅在训练后的前20分钟内引入时才会破坏记忆，而缺氧仅在训练后的前2分钟内给予时才起作用。在记忆巩固的四阶段模型背景下进行解释时，结果表明：1）冷麻醉会破坏短期记忆（STM）和抗麻醉记忆（ARM）；2）缺氧专门破坏ARM；3）两者都使长期记忆（LTM）保持完整；4）LTM可能与果蝇中STM和ARM的可用性无关。
