大肠杆菌中不依赖类核的细胞分裂位点鉴定
Nucleoid-independent identification of cell division sites in Escherichia coli.
作者信息
Cook W R, Rothfield L I
机构信息
Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
出版信息
J Bacteriol. 1999 Mar;181(6):1900-5. doi: 10.1128/JB.181.6.1900-1905.1999.
The mechanism used by Escherichia coli to determine the correct site for cell division is unknown. In this report, we have attempted to distinguish between a model in which septal position is determined by the position of the nucleoids and a model in which septal position is predetermined by a mechanism that does not involve nucleoid position. To do this, filaments with extended nucleoid-free regions adjacent to the cell poles were produced by simultaneous inactivation of cell division and DNA replication. The positions of septa that formed within the nucleoid-free zones after division was allowed to resume were then analyzed. The results showed that septa were formed at a uniform distance from cell poles when division was restored, with no relation to the distance from the nearest nucleoid. In some cells, septa were formed directly over nucleoids. These results are inconsistent with models that invoke nucleoid positioning as the mechanism for determining the site of division site formation.
大肠杆菌用于确定细胞分裂正确位点的机制尚不清楚。在本报告中，我们试图区分两种模型：一种是隔膜位置由类核位置决定的模型，另一种是隔膜位置由不涉及类核位置的机制预先确定的模型。为此，通过同时使细胞分裂和DNA复制失活，产生了在细胞两极附近具有延伸的无类核区域的丝状体。然后分析了恢复分裂后在无类核区域内形成的隔膜的位置。结果表明，恢复分裂时，隔膜在距细胞两极的均匀距离处形成，与距最近类核的距离无关。在一些细胞中，隔膜直接在类核上方形成。这些结果与将类核定位作为确定分裂位点形成机制的模型不一致。
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