Wilkins T D, Chalgren S
Antimicrob Agents Chemother. 1976 Dec;10(6):926-8. doi: 10.1128/AAC.10.6.926.
A medium is described which was designed for use in testing the minimal inhibitory concentration of antibiotics for anaerobic bacteria by agar dilution. It contains: Trypticase (1%), Gelysate (1%), yeast extract (0.5%), glucose (0.1%), pyruvate (0.1%), arginine (0.1%), NaCl (0.5%), hemin (5 mug/ml), vitamin K(1) (0.5 mug/ml), agar (1.5%). The medium does not require the addition of blood to support growth of most clinical isolates of anaerobic bacteria.
本文描述了一种用于通过琼脂稀释法测试抗生素对厌氧菌最低抑菌浓度的培养基。它含有:胰蛋白酶(1%)、溶菌酶(1%)、酵母提取物(0.5%)、葡萄糖(0.1%)、丙酮酸(0.1%)、精氨酸(0.1%)、氯化钠(0.5%)、血红素(5微克/毫升)、维生素K(1)(0.5微克/毫升)、琼脂(1.5%)。该培养基无需添加血液即可支持大多数临床厌氧菌分离株的生长。