Schuster U, Schmalz G, Thonemann B, Mendel N, Metzl C
Department of Operative Dentistry and Periodontology, University of Regensburg, Germany.
J Endod. 2001 Apr;27(4):259-65. doi: 10.1097/00004770-200104000-00004.
SV40 large T-antigen-transfected bovine pulp-derived cells were grown three-dimensionally on polyamide meshes. For optimal cell growth, various cell numbers and mesh coatings were tested. Next the three-dimensional cultures were used in a dentin barrier test device, and the system was evaluated by testing a set of dental filling materials. After 24 hr exposure with or without perfusion of the pulpal part of the test device, cell survival was evaluated using an MTT assay. In all experiments pulp-derived cells transfected with SV40 large T-antigen grew three-dimensionally on polyamide meshes and showed growth kinetics similar to those on cell culture plates with lag, log, and plateau phases (reached after about 14 days of incubation). Cross-sections of the three-dimensional cell cultures revealed about 15 to 20 cell layers. In vitro cytotoxicity tests resulted in cell survival rates which are in good agreement with in vivo data and with results obtained from cytotoxicity tests with three-dimensional cultures of human foreskin fibroblasts.
SV40大T抗原转染的牛牙髓来源细胞在聚酰胺网片上进行三维培养。为了实现最佳细胞生长，对不同的细胞数量和网片涂层进行了测试。接下来，将三维培养物用于牙本质屏障测试装置，并通过测试一组牙科填充材料对该系统进行评估。在对测试装置的牙髓部分进行24小时暴露（有无灌注）后，使用MTT法评估细胞存活率。在所有实验中，用SV40大T抗原转染的牙髓来源细胞在聚酰胺网片上进行三维生长，并表现出与在细胞培养板上相似的生长动力学，包括延迟期、对数期和平稳期（孵育约14天后达到）。三维细胞培养的横截面显示约有15至20个细胞层。体外细胞毒性测试所得的细胞存活率与体内数据以及用人包皮成纤维细胞三维培养进行细胞毒性测试所获得的结果高度一致。
