Bosch R, Moore E R, García-Valdés E, Pieper D H
Departament de Biologia, Microbiologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats, 07071, Palma de Mallorca, Spain.
J Bacteriol. 1999 Apr;181(8):2315-22. doi: 10.1128/JB.181.8.2315-2322.1999.
Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated outside but in close proximity to this transcriptional unit. The nahG and nahW genes of P. stutzeri AN10 are induced and expressed upon incubation with salicylate, and the enzymes that are encoded, NahG and NahW, are involved in naphthalene and salicylate metabolism. Both genes, nahG and nahW, have been cloned in Escherichia coli JM109. The overexpression of these genes yields peptides with apparent molecular masses of 46 kDa (NahG) and 43 kDa (NahW), respectively. Both enzymes exhibit broad substrate specificities and metabolize salicylate, methylsalicylates, and chlorosalicylates. However, the relative rates by which the substituted analogs are transformed differ considerably.
在萘降解菌株斯氏假单胞菌AN10中，已鉴定出两个编码两种独立水杨酸1-羟化酶的基因，即nahG和nahW。虽然nahG与间位裂解途径基因位于同一转录单元中，形成萘降解下游途径，但nahW位于该转录单元之外但与之紧邻。斯氏假单胞菌AN10的nahG和nahW基因在与水杨酸一起培养时被诱导并表达，所编码的酶NahG和NahW参与萘和水杨酸的代谢。nahG和nahW这两个基因都已克隆到大肠杆菌JM109中。这些基因的过表达分别产生表观分子量为46 kDa（NahG）和43 kDa（NahW）的肽。两种酶都表现出广泛的底物特异性，可代谢水杨酸、甲基水杨酸酯和氯水杨酸酯。然而，取代类似物的转化相对速率差异很大。
