MacNeill S R, Cobb C M, Rapley J W, Glaros A G, Spencer P
Department of Periodontics, School of Dentistry, University of Missouri-Kansas City, 64108, USA.
J Clin Periodontol. 1999 Apr;26(4):239-45. doi: 10.1034/j.1600-051x.1999.260407.x.
The purpose of this study was to compare the in vivo osseous healing response of 4 commercially-available synthetic bone grafting materials; hydroxylapatite (HA), calcium sulfate (CaSO4) plus autogenous bone, or a bioactive glass ceramic: with particle size of 300-360 microm (BG1) or 90 to 710 microm (BG2). 4 osteotomy sites were prepared in each tibia of 10 adult male rabbits. One unfilled osteotomy site served as negative control (NC) and another site filled with autogenous bone was the positive control (PC). All animals received BG1 in 2 sites and BG2 in 2 sites. 5 animals received HA and five CaSO4 plus autogenous bone in the remaining 2 sites. Animals were sacrificed at 28 days post-surgery, histologic sections obtained and the % surface area of new bone formation for each material was determined by computerized image analysis. All graft sites showed evidence of bone formation, i.e., (NC) 41.95%; (PC) 50.41%; (BG1) 41.82%; (BG2) 40.36%; (HA) 41.83% and (CaSO4) 58.83%. Statistical analysis using an ANOVA with repeated measures on the materials common to all animals (excluding HA and CaSO4 groups) showed significant differences between materials in surface area of bone, with positive controls better than negative controls, and BG1 and BG2 not significantly different from the negative control. These results indicate that synthetic graft materials can support new bone formation in surgically prepared defects. The utility of a rabbit model for studying physiologic osseous turnover and healing is questioned for studies of slowly resorbing synthetic graft materials.
本研究的目的是比较4种市售合成骨移植材料的体内骨愈合反应；羟基磷灰石（HA）、硫酸钙（CaSO4）加自体骨，或一种生物活性玻璃陶瓷：粒径为300 - 360微米（BG1）或90至710微米（BG2）。在10只成年雄性兔的每只胫骨上制备4个截骨部位。一个未填充的截骨部位作为阴性对照（NC），另一个填充自体骨的部位作为阳性对照（PC）。所有动物在2个部位接受BG1，在2个部位接受BG2。5只动物在其余2个部位接受HA，另外5只接受CaSO4加自体骨。术后28天处死动物，获取组织学切片，并通过计算机图像分析确定每种材料新骨形成的表面积百分比。所有移植部位均显示有骨形成的迹象，即（NC）41.95%；（PC）50.41%；（BG1）41.82%；（BG2）40.36%；（HA）41.83%和（CaSO4）58.83%。对所有动物共有的材料（不包括HA和CaSO4组）进行重复测量方差分析的统计分析表明，材料在骨表面积方面存在显著差异，阳性对照优于阴性对照，BG1和BG2与阴性对照无显著差异。这些结果表明，合成移植材料可以支持手术制备缺损部位的新骨形成。对于研究缓慢吸收的合成移植材料而言，兔模型用于研究生理性骨转换和愈合的实用性受到质疑。
