Calabrò M L, Fiore J R, Favero A, Lepera A, Saracino A, Angarano G, Schulz T F, Chieco-Bianchi L
Department of Oncology and Surgical Sciences, Oncology Section, University of Padova, I-35128 Padova, Italy.
J Infect Dis. 1999 Jun;179(6):1534-7. doi: 10.1086/314765.
Epidemiologic studies suggest that human herpesvirus 8 (HHV-8) may be sexually transmitted. To study the potential for HHV-8 transmission through cervicovaginal (CV) secretions, the presence of HHV-8 DNA was investigated by nested polymerase chain reaction in the cellular fraction of CV secretions from 36 human immunodeficiency virus type 1 (HIV-1)-seropositive and 29 HIV-1-seronegative women. The same patients were tested for antibodies to two defined HHV-8 antigens (latency-associated nuclear antigen and open-reading frame 65-encoded structural protein) and for HHV-8 DNA in their peripheral blood mononuclear cells (PBMC). The findings were compared with the rate of HHV-8 detection in semen samples of 20 HIV-1-infected men. HHV-8 DNA was detected in the CV samples from only 1 HHV-8-seropositive AIDS patient, in 3 PBMC samples (1/29 HIV-1-seronegative patients, 1/3 AIDS patients with Kaposi's sarcoma, and 1/19 AIDS patients), and in 1 of 20 semen samples. HHV-8 infection was more common in HIV-1-infected than uninfected women. Thus HHV-8 DNA is only rarely detectable in CV secretions and semen of HHV-8-infected individuals.
流行病学研究表明，人类疱疹病毒8型（HHV-8）可能通过性传播。为了研究HHV-8通过宫颈阴道（CV）分泌物传播的可能性，采用巢式聚合酶链反应检测了36例1型人类免疫缺陷病毒（HIV-1）血清阳性和29例HIV-1血清阴性女性CV分泌物细胞部分中的HHV-8 DNA。对相同患者检测了针对两种明确的HHV-8抗原（潜伏相关核抗原和开放阅读框65编码的结构蛋白）的抗体以及外周血单核细胞（PBMC）中的HHV-8 DNA。将这些结果与20例HIV-1感染男性精液样本中的HHV-8检测率进行了比较。仅在1例HHV-8血清阳性艾滋病患者的CV样本、3份PBMC样本（1/29例HIV-1血清阴性患者、1/3例患有卡波西肉瘤的艾滋病患者和1/19例艾滋病患者）以及20份精液样本中的1份中检测到HHV-8 DNA。HHV-8感染在HIV-1感染女性中比未感染女性更常见。因此，在HHV-8感染个体的CV分泌物和精液中很少能检测到HHV-8 DNA。
