Pierce C W, Kapp J A
J Exp Med. 1978 Nov 1;148(5):1282-91. doi: 10.1084/jem.148.5.1282.
The ability of spleen cells from (responder X nonresponder)F(1) mice immunized with various GAT-Mphi, GAT-MBSA, and soluble GAT to develop IgG GAT-specific PFC responses in vitro after stimulation with responder and nonresponder parental and F(1) GAT-Mphi, was investigated. F(1) spleen cells from mice immunized with F(1) GAT-Mphi or GAT-MBSA developed secondary responses to responder and nonresponder parental and F(1) GAT- Mphi, but not to unrelated third party GAT-Mphi. Spleen cells from F(1) mice immunized with either parental GAT-Mphi developed secondary responses to F(1) GAT-Mphi and only the parental GAT-Mphi used for immunization in vivo. Soluble GAT-primed F(1) spleen cells responded to F(1) and responder parental, but not nonresponder parental, GAT-Mphi. Simultaneous immunization in vivo with the various GAT-Mphi or GAT-MBSA plus soluble GAT modulated the response pattern of these F(1) spleen cells such that they developed secondary responses only to F(1) and parental responder GAT-Mphi regardless of the response pattern observed after immunization with the various GAT-Mphi or GAT-MBSA alone. These observations demonstrate the critical importance of the physical state of the GAT used for immunization in determining the subsequent response pattern of immune F(1) spleen cells to the parental and F(1) GAT-Mphi. Further, suppressor T cells, capable of inhibiting primary responses to GAT by virgin F(1) spleen cells stimulated by nonresponder parental GAT-Mphi, were demonstrated in spleens of F(1) mice immunized with soluble GAT, but not those primed with F(1) GAT-Mphi. Because responder parental mice develop both helper and suppressor T cells after immunization with GAT-Mphi, and soluble GAT preferentially stimulates suppressor T cells whereas GAT-Mphi stimulate helper T cells in nonresponder parental mice, these observations suggest that distinct subsets of T cells exist in F(1) mice which behave phenotypically as responder and nonresponder parental T cells after immunization with soluble GAT and GAT- Mphi.
研究了用各种谷氨酸 - 巨噬细胞(GAT - Mphi)、谷氨酸 - 牛血清白蛋白(GAT - MBSA)和可溶性谷氨酸免疫的(反应者X无反应者)F1小鼠的脾细胞,在受到反应者和无反应者亲代及F1 GAT - Mphi刺激后,体外产生IgG谷氨酸特异性空斑形成细胞(PFC)反应的能力。用F1 GAT - Mphi或GAT - MBSA免疫的小鼠的F1脾细胞,对反应者和无反应者亲代及F1 GAT - Mphi产生二次反应,但对无关的第三方GAT - Mphi无反应。用亲代GAT - Mphi免疫的F1小鼠的脾细胞,对F1 GAT - Mphi以及仅对体内用于免疫的亲代GAT - Mphi产生二次反应。可溶性谷氨酸致敏的F1脾细胞对F1和亲代反应者的GAT - Mphi有反应,但对亲代无反应者的GAT - Mphi无反应。用各种GAT - Mphi或GAT - MBSA加可溶性谷氨酸同时进行体内免疫,调节了这些F1脾细胞的反应模式,使其仅对F1和亲代反应者的GAT - Mphi产生二次反应,而不管单独用各种GAT - Mphi或GAT - MBSA免疫后观察到的反应模式如何。这些观察结果表明,用于免疫的谷氨酸的物理状态在决定免疫的F1脾细胞对亲代和F1 GAT - Mphi的后续反应模式中至关重要。此外,在用可溶性谷氨酸免疫的F1小鼠的脾脏中证明了抑制性T细胞,其能够抑制由无反应者亲代GAT - Mphi刺激的原始F1脾细胞对谷氨酸的初次反应,但在用F1 GAT - Mphi致敏的小鼠脾脏中未发现。因为反应者亲代小鼠在用GAT - Mphi免疫后会产生辅助性和抑制性T细胞,并且可溶性谷氨酸优先刺激抑制性T细胞,而GAT - Mphi在无反应者亲代小鼠中刺激辅助性T细胞,这些观察结果表明,F1小鼠中存在不同的T细胞亚群,在用可溶性谷氨酸和GAT - Mphi免疫后,其表型行为类似于反应者和亲代无反应者的T细胞。