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体外免疫反应的遗传控制。II. 小鼠脾细胞在体外对随机三聚物1-谷氨酸60-1-丙氨酸30-1-酪氨酸10（GAT）产生原发性噬斑形成细胞反应的细胞条件。

Genetic control of immune responses in vitro. II. Cellular requirements for the development of primary plaque-forming cell responses to the random terpolymer 1-glutamic acid 60-1-alanine30-1-tyrosine10 (GAT) by mouse spleen cells in vitro.

作者信息

Kapp J A, Pierce C W, Benacerraf B

出版信息

J Exp Med. 1973 Nov 1;138(5):1121-32. doi: 10.1084/jem.138.5.1121.

DOI:10.1084/jem.138.5.1121
PMID:4126767
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2139443/
Abstract

The cellular requirements for the development of primary IgG GAT-specific PFC responses in cultures of spleen cells from responder, C57Bl/6, mice stimulated with GAT and GAT-MBSA and in cultures of spleen cells from nonresponder, SJL and B10.S, mice stimulated with GAT-MBSA were investigated. Macrophages were required for development of responses to GAT and GAT-MBSA in cultures of spleen cells from responder mice and for responses to GAT-MBSA in cultures of spleen cells from nonresponder mice. Macrophages from nonresponder mice supported the development of responses to GAT by nonadherent responder spleen cells, indicating that the failure of nonresponder mice to respond to GAT is not due to a macrophage defect. Furthermore, responder macrophages supported the responses of nonadherent, nonresponder spleen cells to SRBC and GAT-MBSA, but not to GAT. This indicates that the capacity to respond to GAT is a function of the nonadherent population which is composed of thymus-derived (T) helper cells and precursors of antibody-producing cells. Treatment of spleen cells with anti-theta serum and complement before culture initiation abolished PFC responses to GAT and GAT-MBSA thus establishing the requirement for T cells in the development of PFC responses to these antigens. Since precursors of antibody-producing cells in nonresponder mice are capable of synthesizing antibody specific for GAT after stimulation with GAT-MBSA and since the response to GAT is thymus-dependent, it appears that nonresponder mice lack GAT-specific helper T cell function.

摘要

研究了用谷氨酸-钥孔戚血蓝蛋白（GAT）和GAT-甲基牛血清白蛋白（GAT-MBSA）刺激的应答小鼠C57Bl/6脾细胞培养物中，以及用GAT-MBSA刺激的无应答小鼠SJL和B10.S脾细胞培养物中，原发性IgG GAT特异性空斑形成细胞（PFC）反应发生的细胞需求。在应答小鼠脾细胞培养物中，巨噬细胞是对GAT和GAT-MBSA产生反应所必需的，在无应答小鼠脾细胞培养物中，巨噬细胞是对GAT-MBSA产生反应所必需的。来自无应答小鼠的巨噬细胞支持非黏附性应答脾细胞对GAT的反应，这表明无应答小鼠对GAT无反应并非由于巨噬细胞缺陷。此外，应答巨噬细胞支持非黏附性、无应答脾细胞对绵羊红细胞（SRBC）和GAT-MBSA的反应，但不支持对GAT的反应。这表明对GAT产生反应的能力是由胸腺来源的（T）辅助细胞和抗体产生细胞前体组成的非黏附群体的功能。在培养开始前用抗θ血清和补体处理脾细胞，消除了对GAT和GAT-MBSA的PFC反应，从而确定了在对这些抗原的PFC反应发生过程中对T细胞的需求。由于无应答小鼠中的抗体产生细胞前体在用GAT-MBSA刺激后能够合成针对GAT的抗体，并且由于对GAT的反应是胸腺依赖性的，因此看来无应答小鼠缺乏GAT特异性辅助性T细胞功能。

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Genetic control of immune responses in vitro. II. Cellular requirements for the development of primary plaque-forming cell responses to the random terpolymer 1-glutamic acid 60-1-alanine30-1-tyrosine10 (GAT) by mouse spleen cells in vitro.体外免疫反应的遗传控制。II. 小鼠脾细胞在体外对随机三聚物1-谷氨酸60-1-丙氨酸30-1-酪氨酸10（GAT）产生原发性噬斑形成细胞反应的细胞条件。
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Haplotype-specific suppression of antibody responses in vitro. I. Generation of genetically restricted suppressor T cells by neonatal treatment with semiallogeneic spleen cells.体外单倍型特异性抗体应答抑制。I. 用半同种异体脾细胞对新生动物进行处理产生基因受限的抑制性T细胞。
J Exp Med. 1981 Jul 1;154(1):35-47. doi: 10.1084/jem.154.1.35.
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Macrophages: modulators of immunity. Parke-Davis Award Lecture.巨噬细胞：免疫调节剂。帕克-戴维斯奖演讲。
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Major histocompatibility complex-controlled, antigen-presenting cell-expressed specificity of T cell antigen recognition. Identification of a site of interaction and its relationship to Ir genes.主要组织相容性复合体控制的、抗原呈递细胞表达的T细胞抗原识别特异性。相互作用位点的鉴定及其与Ir基因的关系。
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Immune responses in vitro. XI. Suppression of primary IgM and IgG plaque-forming cell responses in vitro by alloantisera against leukocyte alloantigens.体外免疫反应。十一、抗白细胞同种抗原的同种抗血清对体外原发性IgM和IgG空斑形成细胞反应的抑制作用。
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Cell interactions between histoincompatible T and B lymphocytes. IV. Involvement of the immune response (Ir) gene in the control of lymphocyte interactions in responses controlled by the gene.组织相容性不相容的T淋巴细胞与B淋巴细胞之间的细胞相互作用。IV. 免疫反应（Ir）基因在由该基因控制的反应中对淋巴细胞相互作用的控制作用。
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A cellular basis for genetically controlled immunologic unresponsiveness in mice: tolerance induction in T-cells.小鼠基因控制的免疫无反应性的细胞基础：T细胞中的耐受性诱导。
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Histocompatibility-linked immune response gene function in guinea pigs. Specific inhibition of antigen-induced lymphocyte proliferation by alloantisera.豚鼠中组织相容性连锁免疫反应基因的功能。同种异体抗血清对抗原诱导的淋巴细胞增殖的特异性抑制。
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Immune responses in vitro. IV. Suppression of primary M, G, and A plaque-forming cell responses in mouse spleen cell cultures by class-specific antibody to mouse immunoglobulins.体外免疫反应。IV. 用小鼠免疫球蛋白的类特异性抗体抑制小鼠脾细胞培养物中初级M、G和A斑块形成细胞反应。
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