体外免疫反应的遗传控制。V. 三元共聚物L-谷氨酸60-L-丙氨酸30-L-酪氨酸10（GAT）对无反应小鼠中抑制性T细胞的刺激
Genetic control of immune responses in vitro. V. Stimulation of suppressor T cells in nonresponder mice by the terpolymer L-glutamic acid 60-L-alanine 30-L-tyrosine 10 (GAT).
作者信息
Kapp J A, Pierce C W, Schlossman S, Benacerraf B
出版信息
J Exp Med. 1974 Sep 1;140(3):648-59. doi: 10.1084/jem.140.3.648.
Abstract
In recent studies we have found that GAT not only fails to elicit a GAT-specific response in nonresponder mice but also specifically decreases the ability of nonresponder mice to develop a GAT-specific PFC response to a subsequent challenge with GAT bound to the immunogenic carrier, MBSA. Studies presented in this paper demonstrate that B cells from nonresponder, DBA/1 mice rendered unresponsive by GAT in vivo can respond in vitro to GAT-MBSA if exogenous, carrier-primed T cells are added to the cultures. The unresponsiveness was shown to be the result of impaired carrier-specific helper T-cell function in the spleen cells of GAT-primed mice. Spleen cells from GAT-primed mice specifically suppressed the GAT-specific PFC response of spleen cells from normal DBA/1 mice incubated with GAT-MBSA. This suppression was prevented by pretreatment of GAT-primed spleen cells with anti-theta serum plus C or X irradiation. Identification of the suppressor cells as T cells was confirmed by the demonstration that suppressor cells were confined to the fraction of the column-purified lymphocytes which contained theta-positive cells and a few non-Ig-bearing cells. The significance of these data to our understanding of Ir-gene regulation of the immune response is discussed.
摘要
在最近的研究中，我们发现，γ-氨基丁酸（GAT）不仅无法在无反应小鼠中引发GAT特异性反应，而且还会特异性地降低无反应小鼠对随后用与免疫原性载体甲基化牛血清白蛋白（MBSA）结合的GAT进行攻击产生GAT特异性的脾小结前体细胞（PFC）反应的能力。本文所展示的研究表明，如果将外源性的、经载体致敏的T细胞添加到培养物中，在体内被GAT诱导为无反应状态的无反应DBA/1小鼠的B细胞在体外能够对GAT-MBSA产生反应。这种无反应状态被证明是GAT致敏小鼠脾细胞中载体特异性辅助性T细胞功能受损的结果。GAT致敏小鼠的脾细胞特异性地抑制了与GAT-MBSA一起孵育的正常DBA/1小鼠脾细胞的GAT特异性PFC反应。用抗θ血清加补体（C）或X射线照射对GAT致敏的脾细胞进行预处理可防止这种抑制作用。通过证明抑制细胞局限于柱纯化淋巴细胞中含有θ阳性细胞和一些非免疫球蛋白携带细胞的部分，证实了抑制细胞为T细胞。本文讨论了这些数据对于我们理解免疫反应的Ir基因调控的意义。
相似文献
1
Genetic control of immune responses in vitro. V. Stimulation of suppressor T cells in nonresponder mice by the terpolymer L-glutamic acid 60-L-alanine 30-L-tyrosine 10 (GAT).体外免疫反应的遗传控制。V. 三元共聚物L-谷氨酸60-L-丙氨酸30-L-酪氨酸10（GAT）对无反应小鼠中抑制性T细胞的刺激
J Exp Med. 1974 Sep 1;140(3):648-59. doi: 10.1084/jem.140.3.648.
2
Suppressor T-cell activity in responder X nonresponder (C57BL/10 X DBA/1)F1 spleen cells responsive to L-glutamic acid60-L-alanine30-L-tyrosine10.对L-谷氨酸60-L-丙氨酸30-L-酪氨酸10有反应的反应者X无反应者（C57BL/10×DBA/1）F1脾细胞中的抑制性T细胞活性
J Exp Med. 1978 Nov 1;148(5):1282-91. doi: 10.1084/jem.148.5.1282.
3
Genetic control of immune responses in vitro. II. Cellular requirements for the development of primary plaque-forming cell responses to the random terpolymer 1-glutamic acid 60-1-alanine30-1-tyrosine10 (GAT) by mouse spleen cells in vitro.体外免疫反应的遗传控制。II. 小鼠脾细胞在体外对随机三聚物1-谷氨酸60-1-丙氨酸30-1-酪氨酸10（GAT）产生原发性噬斑形成细胞反应的细胞条件。
J Exp Med. 1973 Nov 1;138(5):1121-32. doi: 10.1084/jem.138.5.1121.
4
Genetic control of immune responses in vitro. 3. Tolerogenic properties of the terpolymer L-glutamic acid 60-L-alanine30-L-tyrosine10 (GAT) for spleen cells from nonresponder (H-2s and H-2q) mice.体外免疫反应的遗传控制。3. 三元共聚物L-谷氨酸60-L-丙氨酸30-L-酪氨酸10（GAT）对无反应性（H-2s和H-2q）小鼠脾细胞的致耐受性特性。
J Exp Med. 1974 Jul 1;140(1):172-84. doi: 10.1084/jem.140.1.172.
5
Genetic control of immune responses in vitro. VI. Experimental conditions for the development of helper T-cell activity specific for the terpolymer L-glutamic aicd60-L-alanine30-L-tyrosine10 (GAT) in nonresponder mice.体外免疫反应的遗传控制。VI. 无反应小鼠中针对三元共聚物L-谷氨酸60-L-丙氨酸30-L-酪氨酸10（GAT）的辅助性T细胞活性发育的实验条件。
J Exp Med. 1975 Jul 1;142(1):50-60. doi: 10.1084/jem.142.1.50.
6
Immunosuppressive factors from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10. IV. Lack of strain restrictions among allogeneic, nonresponder donors and recipients.用L-谷氨酸60-L-丙氨酸30-L-酪氨酸10致敏的无反应小鼠淋巴细胞产生的免疫抑制因子。IV. 同种异体无反应供体和受体之间不存在品系限制。
J Exp Med. 1978 Apr 1;147(4):997-1006. doi: 10.1084/jem.147.4.997.
7
Genetic control of specific immune suppression. IV. Responsiveness to the random copolymer L-glutamic acid50-L-tyrosine50 induced in BALB/c mice by cyclophosphamide.特异性免疫抑制的遗传控制。IV. 环磷酰胺诱导BALB/c小鼠对随机共聚物L-谷氨酸50-L-酪氨酸50的反应性。
J Exp Med. 1976 Jul 1;144(1):277-81. doi: 10.1084/jem.144.1.277.
8
Genetic control of immune responses in vitro. I. Development of primary and secondary plaque-forming cell responses to the random terpolymer 1-glutamic acid 60-1-alanine30-1-tyrosine10 (GAT) by mouse spleen cells in vitro.体外免疫反应的遗传控制。I. 小鼠脾细胞在体外对随机三元共聚物1-谷氨酸60-1-丙氨酸30-1-酪氨酸10（GAT）的初次和二次噬斑形成细胞反应的发展。
J Exp Med. 1973 Nov 1;138(5):1107-20. doi: 10.1084/jem.138.5.1107.
9
The involvement of suppressor T cells in Ir gene regulation of secondary antibody responses of primed (responder X nonresponder)F1 mice to macrophage-bound L-glutamic acid60-L-alanine30-L-tyrosine.抑制性T细胞参与致敏（反应者×无反应者）F1小鼠对巨噬细胞结合的L-谷氨酸60-L-丙氨酸30-L-酪氨酸二次抗体反应的Ir基因调控。
J Exp Med. 1978 Nov 1;148(5):1324-37. doi: 10.1084/jem.148.5.1324.
10
Antigen-specific T-cell-mediated suppression. I. Induction of L-glutamic acid60-L-alanine30-L-tyrosine10 specific suppressor T cells in vitro requires both antigen-specific T-cell-suppressor factor and antigen.抗原特异性T细胞介导的抑制作用。I. 体外诱导L-谷氨酸60-L-丙氨酸30-L-酪氨酸10特异性抑制性T细胞需要抗原特异性T细胞抑制因子和抗原。
J Exp Med. 1978 Jan 1;147(1):123-36. doi: 10.1084/jem.147.1.123.
引用本文的文献
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Special regulatory T-cell review: A rose by any other name: from suppressor T cells to Tregs, approbation to unbridled enthusiasm.特殊调节性T细胞综述：换个名字的玫瑰：从抑制性T细胞到调节性T细胞，从认可到过度热情。
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A T cell receptor antagonist peptide induces T cells that mediate bystander suppression and prevent autoimmune encephalomyelitis induced with multiple myelin antigens.一种T细胞受体拮抗剂肽可诱导介导旁观者抑制的T细胞，并预防由多种髓鞘抗原诱导的自身免疫性脑脊髓炎。
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Dual genetic regulation by H-2 and non-H-2-linked genes of the magnitude and isotypic pattern of the dinitrophenyl antibody response to DNP-GAT conjugate.H-2和非H-2连锁基因对二硝基苯基抗体针对二硝基苯基-谷氨酸-丙氨酸-酪氨酸（DNP-GAT）偶联物反应的强度和同型模式的双重遗传调控。
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本文引用的文献
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Immunization of dissociated spleen cell cultures from normal mice.对来自正常小鼠的脾细胞解离培养物进行免疫接种。
J Exp Med. 1967 Sep 1;126(3):423-42. doi: 10.1084/jem.126.3.423.
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Immune responses in vitro. 3. Development of primary gamma-M, gamma-G, and gamma-A plaque-forming cell responses in mouse spleen cell cultures stimulated with heterologous erythrocytes.体外免疫反应。3. 用异种红细胞刺激小鼠脾细胞培养物中主要γ-M、γ-G和γ-A斑块形成细胞反应的发展。
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Genetic control of immune responses in vitro. 3. Tolerogenic properties of the terpolymer L-glutamic acid 60-L-alanine30-L-tyrosine10 (GAT) for spleen cells from nonresponder (H-2s and H-2q) mice.体外免疫反应的遗传控制。3. 三元共聚物L-谷氨酸60-L-丙氨酸30-L-酪氨酸10（GAT）对无反应性（H-2s和H-2q）小鼠脾细胞的致耐受性特性。
J Exp Med. 1974 Jul 1;140(1):172-84. doi: 10.1084/jem.140.1.172.
4
5
Selective roles of thymus-derived lymphocytes in the antibody response. II. Preferential suppression of high-affinity antibody-forming cells by carrier-primed suppressor T cells.胸腺来源淋巴细胞在抗体应答中的选择性作用。II. 载体致敏抑制性T细胞对高亲和力抗体形成细胞的优先抑制作用。
J Exp Med. 1974 Jul 1;140(1):253-66. doi: 10.1084/jem.140.1.253.
6
Genetic control of immune responses in vitro. IV. Conditions for cooperative interactions between nonresponder parental B cells and primed (responder plus nonresponder) F1 T cells in the development of an antibody response under Ir gene control in vitro.体外免疫反应的遗传控制。IV. 在体外Ir基因控制下抗体反应发生过程中，无反应亲代B细胞与致敏（有反应加无反应）F1 T细胞之间协同相互作用的条件。
J Exp Med. 1974 Jul 1;140(1):185-98. doi: 10.1084/jem.140.1.185.
7
Specific purification of lymphocyte populations on a digestible immunoabsorbent.在可消化免疫吸附剂上对淋巴细胞群体进行特异性纯化。
J Immunol. 1973 Jan;110(1):313-9.
8
A cellular basis for genetically controlled immunologic unresponsiveness in mice: tolerance induction in T-cells.小鼠基因控制的免疫无反应性的细胞基础：T细胞中的耐受性诱导。
Proc Natl Acad Sci U S A. 1973 Jan;70(1):250-4. doi: 10.1073/pnas.70.1.250.
9
Radioresistance of carrier-specific helper thymus-derived lymphocytes in mice.小鼠中载体特异性辅助性胸腺来源淋巴细胞的辐射抗性
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3453-8. doi: 10.1073/pnas.69.11.3453.
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