新生儿胆汁淤积症患者Guthrie卡片上巨细胞病毒DNA检测
Cytomegalovirus DNA detection on Guthrie cards in patients with neonatal cholestasis.
作者信息
Fischler B, Rodensjö P, Nemeth A, Forsgren M, Lewensohn-Fuchs I
机构信息
Department of Paediatrics, Huddinge University Hospital, Karolinska Institute, Sweden.
出版信息
Arch Dis Child Fetal Neonatal Ed. 1999 Mar;80(2):F130-4. doi: 10.1136/fn.80.2.f130.
AIM
To time the onset of cytomegalovirus (CMV) infection in patients (n = 39) with CMV associated neonatal cholestasis by analysing CMV DNA on Guthrie cards sampled at 3 days of age.
METHODS
CMV infection was diagnosed by serology/urine isolation or by CMV DNA detection (polymerase chain reaction) in liver biopsy specimens. In order to time the infection dry blood filter paper discs were punched out from stored Guthrie cards. After phenol-choloroform extraction CMV DNA was detected by nested polymerase chain reaction.
RESULTS
All cards from control children (n = 8) with congenital CMV tested positive; none of the negative controls (n = 4) did so. Two of 39 cholestatic infants were CMV DNA positive; their mothers had serological signs compatible with infection during the second half of the pregnancy. All other cholestatic infants tested negative.
CONCLUSIONS
CMV DNA was not detected in most of the children using Guthrie cards, suggesting that infection developed at or soon after birth.
目的
通过分析出生3天时采集于古思里卡片上的样本中的巨细胞病毒（CMV）DNA，确定39例患有CMV相关新生儿胆汁淤积症的患者中CMV感染的起始时间。
方法
通过血清学/尿液分离或肝活检标本中的CMV DNA检测（聚合酶链反应）诊断CMV感染。为了确定感染时间，从储存的古思里卡片上冲压下干血滤纸圆盘。经过酚-氯仿提取后，通过巢式聚合酶链反应检测CMV DNA。
结果
所有先天性CMV感染的对照儿童（n = 8）的卡片检测均呈阳性；所有阴性对照（n = 4）均未检测到阳性。39例胆汁淤积婴儿中有2例CMV DNA呈阳性；他们的母亲在妊娠后半期有与感染相符的血清学迹象。所有其他胆汁淤积婴儿检测均为阴性。
结论
使用古思里卡片在大多数儿童中未检测到CMV DNA，提示感染在出生时或出生后不久发生。
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