Yamamoto Y, Shimamoto K, Sofikitis N, Miyagawa I
Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan.
Hum Reprod. 1999 Jun;14(6):1516-21. doi: 10.1093/humrep/14.6.1516.
The effects of hypercholesterolaemia on testicular endocrine and exocrine function were evaluated. The influence of hypercholesterolaemia on sperm quality, quantity, and fertilizing potential was also determined. Ten mature rabbits (group A) were fed chow containing 3% cholesterol for 12 weeks. Ten control rabbits (group B) were fed normal chow for the same period. At the end of the experimental period testosterone profiles and sperm parameters were evaluated and the sperm reproductive potential was assessed by in vitro fertilization (IVF) techniques. Peripheral serum testosterone responses to testicular stimulation with human chorionic gonadotrophin, androgen-binding protein activity in testicular cytosols, sperm concentration, sperm motility, length of sperm midpiece, and IVF outcome were all significantly lower in group A than in group B. In contrast, serum cholesterol concentrations were significantly higher in group A. There were no significant differences in either testicular versus intra-abdominal temperature differences or cholesterol concentrations in seminal plasma or testicular tissue between groups A and B. The results suggest that hypercholesterolaemia has a detrimental effect on Leydig and Sertoli cell secretory function, spermatogenesis, epididymal sperm maturation process, and the overall sperm fertilizing capacity.
评估了高胆固醇血症对睾丸内分泌和外分泌功能的影响。还确定了高胆固醇血症对精子质量、数量和受精潜力的影响。十只成年兔子（A组）喂食含3%胆固醇的食物12周。十只对照兔子（B组）在同一时期喂食正常食物。在实验期结束时，评估睾酮水平和精子参数，并通过体外受精（IVF）技术评估精子的生殖潜力。A组外周血清睾酮对人绒毛膜促性腺激素刺激睾丸的反应、睾丸胞质溶胶中的雄激素结合蛋白活性、精子浓度、精子活力、精子中段长度和IVF结果均显著低于B组。相比之下，A组血清胆固醇浓度显著更高。A组和B组在睾丸与腹腔内温度差异、精浆或睾丸组织中的胆固醇浓度方面均无显著差异。结果表明，高胆固醇血症对睾丸间质细胞和支持细胞的分泌功能、精子发生、附睾精子成熟过程以及精子的整体受精能力有不利影响。
