Green R, Simpson A, Custovic A, Faragher B, Chapman M, Woodcock A
North West Lung Centre, Wythenshawe Hospital, Manchester, UK.
Allergy. 1999 May;54(5):484-8. doi: 10.1034/j.1398-9995.1999.00029.x.
Effective methods of reducing dog allergen are required to help alleviate symptoms in asthmatic patients sensitized to dog who refuse to part with their pet. The aim of this study was to investigate the use of the high efficiency particulate air (HEPA) filter air cleaner to reduce airborne Can f 1 in homes with a dog.
The effect of a HEPA air cleaner was investigated in nine homes with a dog. Samples were collected from two rooms of each house concurrently, one of which contained the dog, on two separate days (active day - HEPA air cleaner on - and control day). Eight consecutive 1-h samples were collected from each room with a high-volume air sampler (airflow rate 60 l/ min). Can f 1 was determined by monoclonal-polyclonal antibody-based ELISA.
Baseline airborne Can f 1 levels were 3.8-fold greater when sampling was performed with a dog in the room (GM 27.1 ng Can f 1/m3, range 2.63-329) than when the dog was elsewhere in the house (GM 7.1 ng Can f 1/m3, range 0.69-27.2). When the dog was elsewhere in the house, airborne Can f 1 levels fell on both active and control days, but the magnitude of the reduction was significantly greater on the active days (P<0.05), and was approximately 90% from baseline. With the dog in the room, a significant fall in airborne Can f 1 was observed only on active days (75% from baseline), but not on control days (active vs control P<0.001).
HEPA air cleaners reduce airborne Can f 1 in homes with dogs. Furthermore, preventing the access of the dog to the bedroom and possibly the living room may reduce the total allergen load inhaled.
需要有效的减少犬过敏原的方法，以帮助缓解对狗过敏但又拒绝舍弃宠物的哮喘患者的症状。本研究的目的是调查使用高效空气过滤器（HEPA）空气净化器来降低有狗的家庭中空气中犬小孢子菌1（Can f 1）的含量。
在九个有狗的家庭中研究了HEPA空气净化器的效果。在两个不同的日子（使用日——HEPA空气净化器开启——和对照日），同时从每个房屋的两个房间采集样本，其中一个房间有狗。使用大容量空气采样器（流速60升/分钟）从每个房间连续采集八个1小时的样本。通过基于单克隆-多克隆抗体的ELISA法测定Can f 1。
当在有狗的房间采样时，空气中Can f 1的基线水平比狗在房屋其他地方时高3.8倍（几何均值27.1纳克Can f 1/立方米，范围2.63 - 329）（几何均值7.1纳克Can f 1/立方米，范围0.69 - 27.2）。当狗在房屋其他地方时，空气中Can f 1水平在使用日和对照日均下降，但使用日下降幅度显著更大（P<0.05），且从基线下降约90%。当狗在房间时，仅在使用日观察到空气中Can f 1显著下降（从基线下降75%），而在对照日未下降（使用日与对照日相比P<0.001）。
HEPA空气净化器可降低有狗家庭中空气中的Can f 1。此外，防止狗进入卧室以及可能的客厅，可能会减少吸入的总过敏原负荷。
