Gustafsson A, Berstad A, Lund-Tønnesen S, Midtvedt T, Norin E
Dept. of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
Scand J Gastroenterol. 1999 Jun;34(6):580-6. doi: 10.1080/003655299750026038.
Patients with antibiotic-associated diarrhoea (AAD) show significant disturbances in short-chain fatty acid pattern. In the present study five more microflora-associated characteristics (MACs) were investigated before and after administration of an enema containing faecal microflora from a healthy person on a Western diet.
The functions of the microflora were determined with gas chromatography, electrophoresis, and spectrophotometry.
The conversion of cholesterol to coprostanol and the concentration of urobilinogen and trypsin were significantly reduced in comparison with healthy persons. The pattern of mucin was altered, but beta-aspartylglycine remained the same as in healthy persons. Enema treatment influenced these functions to different extents.
Most MACs were significantly disturbed in patients with AAD. Administration of a human faecal enema modified these changes and relieved diarrhoea, usually within 4 days.
抗生素相关性腹泻（AAD）患者的短链脂肪酸模式存在显著紊乱。在本研究中，对食用西方饮食的健康人粪便微生物群灌肠前后的另外五个微生物群相关特征（MACs）进行了调查。
用气相色谱法、电泳法和分光光度法测定微生物群的功能。
与健康人相比，胆固醇向粪甾烷醇的转化以及尿胆原和胰蛋白酶的浓度显著降低。粘蛋白模式发生改变，但β-天冬氨酰甘氨酸与健康人相同。灌肠治疗对这些功能有不同程度的影响。
AAD患者的大多数MACs存在显著紊乱。给予人粪便灌肠可改变这些变化并缓解腹泻，通常在4天内。
