Hukriede N A, Joly L, Tsang M, Miles J, Tellis P, Epstein J A, Barbazuk W B, Li F N, Paw B, Postlethwait J H, Hudson T J, Zon L I, McPherson J D, Chevrette M, Dawid I B, Johnson S L, Ekker M
Laboratory of Molecular Genetics and Unit on Biological Computation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9745-50. doi: 10.1073/pnas.96.17.9745.
The zebrafish is an excellent genetic system for the study of vertebrate development and disease. In an effort to provide a rapid and robust tool for zebrafish gene mapping, a panel of radiation hybrids (RH) was produced by fusion of irradiated zebrafish AB9 cells with mouse B78 cells. The overall retention of zebrafish sequences in the 93 RH cell lines that constitute the LN54 panel is 22%. Characterization of the LN54 panel with 849 simple sequence length polymorphism markers, 84 cloned genes and 122 expressed sequence tags allowed the production of an RH map whose total size was 11,501 centiRays. From this value, we estimated the average breakpoint frequency of the LN54 RH panel to correspond to 1 centiRay = 148 kilobase. Placement of a group of 235 unbiased markers on the RH map suggests that the map generated for the LN54 panel, at present, covers 88% of the zebrafish genome. Comparison of marker positions in RH and meiotic maps indicated a 96% concordance. Mapping expressed sequence tags and cloned genes by using the LN54 panel should prove to be a valuable method for the identification of candidate genes for specific mutations in zebrafish.
斑马鱼是用于研究脊椎动物发育和疾病的优秀遗传系统。为了提供一种快速且强大的斑马鱼基因定位工具，通过将经辐射的斑马鱼AB9细胞与小鼠B78细胞融合，构建了一组辐射杂种（RH）。构成LN54组的93个RH细胞系中斑马鱼序列的总体保留率为22%。用849个简单序列长度多态性标记、84个克隆基因和122个表达序列标签对LN54组进行表征，使得能够构建一个总大小为11,501厘雷的RH图谱。根据这个值，我们估计LN54 RH组的平均断点频率相当于1厘雷 = 148千碱基。在RH图谱上放置一组235个无偏倚标记表明，目前为LN54组生成的图谱覆盖了斑马鱼基因组的88%。RH图谱和减数分裂图谱中标记位置的比较表明一致性为96%。利用LN54组对表达序列标签和克隆基因进行定位，将被证明是鉴定斑马鱼特定突变候选基因的一种有价值的方法。
