Schneider P M, Seo Y, Rittner C
Institute of Legal Medicine, Johannes Gutenberg University, Am Pulverturm 3, D-55131 Mainz, Germany.
Int J Legal Med. 1999;112(5):315-6. doi: 10.1007/s004140050257.
A dog was suspected of having caused a traffic accident. Three hair fragments were recovered from the damaged car and subjected to DNA sequence analysis of the canine mitochondrial D-loop control region. The results were compared to saliva and hair samples from the alleged dog, as well as to control hair samples from four unrelated dogs of different breeds. Two sequence types exhibiting five nucleotide differences in a 377 bp fragment were identified among the four controls. Whereas the evidence hair fragment was identical to the type 1 control sequence, the alleged dog shared the type 2 control sequence except for one position. Thus the dog could be excluded as the origin of the hair fragment. As canine mtDNA appears to exhibit only limited polymorphism, mitochondrial D-loop sequence comparison is currently only suitable for exclusions.
一只狗被怀疑引发了一起交通事故。从受损汽车上提取了三根毛发碎片，并对犬线粒体D-loop控制区进行了DNA序列分析。将结果与被指控的狗的唾液和毛发样本进行了比较，同时也与来自四只不同品种无关犬只的对照毛发样本进行了比较。在四个对照样本中鉴定出两种序列类型，在一个377 bp的片段中存在五个核苷酸差异。而证据毛发碎片与1型对照序列相同，被指控的狗除一个位置外与2型对照序列相同。因此，可以排除这只狗是毛发碎片的来源。由于犬线粒体DNA似乎仅表现出有限的多态性，线粒体D-loop序列比较目前仅适用于排除。
