Ascoli C, Barbi M, Frediani C, Murè A
Biophys J. 1978 Dec;24(3):585-99. doi: 10.1016/S0006-3495(78)85406-X.
Measurements of Euglena gracilis motion parameters have been performed by the spectral analysis of the scattered laser light. Samples were oriented by a radiofrequency field to obtain easily interpretable spectra. Cell rotation frequency and flagellar beating frequency distributions were obtained from the homodyne spectra, whereas the Doppler lines obtained at small observation angles by heterodyne detection yielded the swimming speed distributions. We discuss the broadening of the heterodyne spectra at large angles of observation. An application of this method to the study of the photo-kinetic effect is also described.
通过对散射激光进行光谱分析，测量了纤细裸藻的运动参数。样品通过射频场进行定向，以获得易于解释的光谱。细胞旋转频率和鞭毛摆动频率分布从外差光谱中获得，而异频检测在小观察角度获得的多普勒线则产生了游动速度分布。我们讨论了在大观察角度下外差光谱的展宽。还描述了该方法在光动力学效应研究中的应用。
