Boon J P, Nossal R, Chien S H
Biophys J. 1974 Nov;14(11):847-64. doi: 10.1016/s0006-3495(74)85954-0.
Simple models are used to calculate the inelastic light scattering spectrum of motile bacteria when wiggling motions are included in addition to translational displacement. Computations of spectra lead to the conclusion that nontranslational motions can be neglected when swimming speeds are deduced from light-scattering data for normal vigorously motile strains. On the other hand, for slowly translating bacteria, or for strains exhibiting noticeable wiggling motion when viewed in a microscope, additional spectral components may be significant. Such components are best distinguished when measurements are made at small and intermediate scattering angles; at large angles the spectra have approximately the same scaling properties (functionals of Qt, Q being the Bragg wave vector) as those associated with simple translational motility.
当除了平移位移之外还包括摆动运动时,使用简单模型来计算运动细菌的非弹性光散射光谱。光谱计算得出的结论是,当从正常活跃运动菌株的光散射数据推断游泳速度时,非平移运动可以忽略不计。另一方面,对于平移缓慢的细菌,或者对于在显微镜下观察时表现出明显摆动运动的菌株,额外的光谱成分可能很重要。当在小散射角和中散射角进行测量时,这些成分最容易区分;在大角度时,光谱具有与简单平移运动相关的光谱大致相同的标度特性(Qt的泛函,Q为布拉格波矢)。