糖尿病中的视黄醇、α-生育酚和类胡萝卜素。

Retinol, alpha-tocopherol and carotenoids in diabetes.

作者信息

Abahusain M A, Wright J, Dickerson J W, de Vol E B

机构信息

Department of Family and Community Medicine, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

出版信息

Eur J Clin Nutr. 1999 Aug;53(8):630-5. doi: 10.1038/sj.ejcn.1600825.

DOI:10.1038/sj.ejcn.1600825

PMID:10477250

Abstract

OBJECTIVE

A case-control study was conducted to evaluate the effects of diabetes mellitus on serum levels of vitamin A, alpha-carotene, beta-carotene, alpha-tocopherol, serum and urine RBP.

SUBJECTS

One hundred and seven patients with Type 2 diabetes mellitus (28-74 y) were recruited from those attending a primary health care clinic in King Khalid University Hospital in Riyadh City (Saudi Arabia). They were matched for age and sex with 143 healthy individuals.

METHODS

Fasting blood samples and 10h urine collections were obtained from all subjects. Levels of vitamins and carotenoids in serum measured by high performance liquid chromatography (HPLC), and of retinol binding protein (RBP) in serum and urine by an enzyme-linked immunosorbent assay (ELISA).

RESULTS

The mean serum concentrations of retinol, alpha-carotene, and alpha-tocopherol were similar in both groups after correction of lipid soluble vitamins for serum lipids levels. However, serum beta-carotene concentration was significantly higher in control subjects than diabetics (P = 0.002). Serum and urine RBP concentrations were significantly higher in diabetics than in controls (P = 0.0001). In normal subjects (but not diabetics) serum concentrations of retinol and RBP were higher in men than in women (P = 0.02, P = 0.0001 respectively). In both normal and diabetic subjects, serum levels of alpha-tocopherol (P = 0.007) and urine RBP (P = 0.005), were higher in men than women. Urinary excretion of RBP was significantly higher in diabetic patients with renal impairment than other diabetics or controls (P = 0.0001). There was a negative correlation between fasting blood glucose (FBG) concentration and serum beta-carotene (P = 0.008) in the total combined group and a positive correlation between FBG and urinary RBP/creatinine (P = 0.009) in diabetic patients.

CONCLUSION

Serum beta-carotene concentration was significantly lower in diabetic patients than controls. Serum retinol concentration in patients with diabetes was normal, yet serum and urine RBP concentrations were significantly higher in diabetics than in controls.

摘要

目的

开展一项病例对照研究，以评估糖尿病对血清视黄醇、α-胡萝卜素、β-胡萝卜素、α-生育酚、血清及尿液视黄醇结合蛋白（RBP）水平的影响。

对象

从利雅得市（沙特阿拉伯）哈立德国王大学医院的一家初级保健诊所就诊的患者中招募了107例2型糖尿病患者（年龄28 - 74岁）。他们在年龄和性别上与143名健康个体相匹配。

方法

采集所有受试者的空腹血样和10小时尿液样本。血清中的维生素和类胡萝卜素水平通过高效液相色谱法（HPLC）测定，血清和尿液中的视黄醇结合蛋白（RBP）通过酶联免疫吸附测定法（ELISA）测定。

结果

校正脂溶性维生素的血清脂质水平后，两组的视黄醇、α-胡萝卜素和α-生育酚的平均血清浓度相似。然而，对照组受试者的血清β-胡萝卜素浓度显著高于糖尿病患者（P = 0.002）。糖尿病患者的血清和尿液RBP浓度显著高于对照组（P = 0.0001）。在正常受试者中（但糖尿病患者并非如此），男性的血清视黄醇和RBP浓度高于女性（分别为P = 0.02，P = 0.0001）。在正常和糖尿病受试者中，男性的血清α-生育酚水平（P = 0.007）和尿液RBP水平（P = 0.005）均高于女性。肾功能受损的糖尿病患者的RBP尿排泄量显著高于其他糖尿病患者或对照组（P = 0.0001）。在整个合并组中，空腹血糖（FBG）浓度与血清β-胡萝卜素呈负相关（P = 0.008），在糖尿病患者中，FBG与尿RBP/肌酐呈正相关（P = 0.009）。