耐万古霉素肠球菌的快速检测
Rapid detection of vancomycin-resistant enterococci.
作者信息
Edberg S C, Hardalo C J, Kontnick C, Campbell S
机构信息
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
出版信息
J Clin Microbiol. 1994 Sep;32(9):2182-4. doi: 10.1128/jcm.32.9.2182-2184.1994.
Abstract
Campylobacter blood agar with clindamycin incubated in 6% CO2 served as a medium to both screen for vancomycin resistance and select for presumptive enterococci. Colonies that grew on the medium were specifically identified as enterococci within 30 min by the pyroglutamyl-beta-naphthylamide and rapid bile esculin tests. The combination of a selective medium plus rapid enzyme substrate tests offered an inexpensive means to enumerate vancomycin-resistant enterococci from specimens by using readily available reagents.
摘要
含有克林霉素的弯曲杆菌血琼脂在6%二氧化碳环境中培养，用作筛选耐万古霉素和选择推测性肠球菌的培养基。在该培养基上生长的菌落通过焦谷氨酸-β-萘酰胺和快速胆汁七叶苷试验在30分钟内被明确鉴定为肠球菌。选择性培养基与快速酶底物试验相结合，提供了一种使用现成试剂从标本中计数耐万古霉素肠球菌的廉价方法。
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