Kilbane J J, Ranganathan R, Cleveland L, Kayser K J, Ribiero C, Linhares M M
Institute of Gas Technology, Des Plaines, Illinois 60018, USA.
Appl Environ Microbiol. 2000 Feb;66(2):688-93. doi: 10.1128/AEM.66.2.688-693.2000.
Enrichment culture experiments employing soil and water samples obtained from petroleum-contaminated environments succeeded in the isolation of a pure culture possessing the ability to utilize quinoline as a sole nitrogen source but did not utilize quinoline as a carbon source. This culture was identified as Pseudomonas ayucida based on a partial 16S rRNA gene sequence, and the strain was given the designation IGTN9m. Examination of metabolites using thin-layer chromatography and gas chromatography-mass spectrometry suggests that P. ayucida IGTN9m converts quinoline to 2-quinolinone and subsequently to 8-hydroxycoumarin. Resting cells of P. ayucida IGTN9m were shown to be capable of selectively removing about 68% of quinoline from shale oil in a 16-h treatment time. These results suggest that P. ayucida IGTN9m may be useful in petroleum biorefining for the selective removal of organically bound nitrogen from petroleum.
利用从石油污染环境中获取的土壤和水样进行的富集培养实验成功分离出一种纯培养物，该培养物能够以喹啉作为唯一氮源，但不能将喹啉作为碳源。基于部分16S rRNA基因序列，该培养物被鉴定为阿尤西达假单胞菌（Pseudomonas ayucida），该菌株被命名为IGTN9m。使用薄层色谱法和气相色谱-质谱联用仪对代谢产物进行检测表明，阿尤西达假单胞菌IGTN9m将喹啉转化为2-喹啉酮，随后再转化为8-羟基香豆素。在16小时的处理时间内，阿尤西达假单胞菌IGTN9m的静止细胞能够从页岩油中选择性去除约68%的喹啉。这些结果表明，阿尤西达假单胞菌IGTN9m在石油生物精炼中对于从石油中选择性去除有机结合氮可能是有用的。
