Kerawala C J, Beale V, Reed M, Martin I C
Department of Oral and Facial Surgery, Sunderland Royal Hospital, UK.
Int J Oral Maxillofac Surg. 2000 Feb;29(1):32-5.
Since vital staining has been advocated as a sensitive method of displaying epithelial atypia, 14 oral squamous cell carcinomas were stained immediately preoperatively with toluidine blue and then resected 1 cm outside clinically abnormal or positively staining tissue. The integrity of the entire tissue margin was histologically examined in each case. Whilst vital staining delineated all 14 invasive carcinomas at the centre of each resected specimen, 10 foci of carcinoma-in-situ or severe dysplasia were identified not to have stained at the resection margins. Toluidine blue may, therefore, be an adjunct in identifying invasive tumour at mucosal resection margins. However, it would appear to be of no benefit in delineating positive resection margins due to carcinoma-in-situ or severe dysplasia, and hence it may be of little value in reducing the incidence of local recurrences.
由于活组织染色一直被认为是显示上皮异型性的一种敏感方法，因此对14例口腔鳞状细胞癌在术前即刻用甲苯胺蓝进行染色，然后在临床异常或阳性染色组织外1厘米处进行切除。对每例病例的整个组织边缘的完整性进行了组织学检查。虽然活组织染色在每个切除标本的中心勾勒出了所有14例浸润性癌，但在切除边缘发现有10个原位癌或重度发育异常灶未被染色。因此，甲苯胺蓝可能有助于识别黏膜切除边缘的浸润性肿瘤。然而，它似乎对勾勒出因原位癌或重度发育异常导致的阳性切除边缘没有帮助，因此在降低局部复发率方面可能价值不大。
