Sokol P A, Kooi C, Hodges R S, Cachia P, Woods D E
Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1.
J Infect Dis. 2000 May;181(5):1682-92. doi: 10.1086/315470. Epub 2000 May 15.
Pseudomonas aeruginosa and Burkholderia cepacia produce metalloproteases that effect lung injury. Two epitopes (peptides 15 and 42) previously identified on P. aeruginosa elastase induce the production of antibodies that neutralize protease activity. The effects of immunization with synthetic peptides based on these epitopes on experimental lung infections due to P. aeruginosa or B. cepacia were examined. Rats were immunized with peptides conjugated to keyhole limpet hemocyanin or tetanus toxoid before infection. Immunization with peptide 15 (pep15) resulted in a decrease in total cells and polymorphonuclear leukocytes in bronchoalveolar lavage (BAL) fluid and a 50%-70% decrease in lung histopathologic changes, compared with findings in controls. Immunization with peptide 42 decreased cells in BAL fluid but did not decrease lung pathologic changes. Immunization with pep15 alone was just as effective in protecting against lung injury as immunization with a combination of both peptides. These studies suggest that immunization with pep15 can reduce the severity of lung infections due to P. aeruginosa or B. cepacia.
铜绿假单胞菌和洋葱伯克霍尔德菌可产生导致肺损伤的金属蛋白酶。先前在铜绿假单胞菌弹性蛋白酶上鉴定出的两个表位（肽段15和42）可诱导产生中和蛋白酶活性的抗体。研究了基于这些表位的合成肽免疫对铜绿假单胞菌或洋葱伯克霍尔德菌所致实验性肺部感染的影响。在感染前，用与钥孔戚血蓝蛋白或破伤风类毒素偶联的肽对大鼠进行免疫。与对照组相比，用肽段15（pep15）免疫可使支气管肺泡灌洗（BAL）液中的总细胞数和多形核白细胞减少，肺组织病理学变化减少50%-70%。用肽段42免疫可使BAL液中的细胞减少，但未减轻肺部病理变化。单独用pep15免疫在预防肺损伤方面与用两种肽联合免疫同样有效。这些研究表明，用pep15免疫可减轻铜绿假单胞菌或洋葱伯克霍尔德菌所致肺部感染的严重程度。
