Wang S p
Department of Pathobiology, University of Washington, Seattle, WA 98195, USA.
J Infect Dis. 2000 Jun;181 Suppl 3:S421-5. doi: 10.1086/315622.
A brief description of current procedures for the Chlamydia microimmunofluorescence (MIF) test is presented. To date, use of MIF serology with Chlamydia pneumoniae (TWAR) antigen has provided the most sensitive and specific method for diagnosis of acute TWAR infection. In primary infections, the TWAR IgM antibody response is longer lasting and IgG antibody is slower to develop compared with Chlamydia trachomatis infection. Unlike other Chlamydia species, only a single serovar for C. pneumoniae has been recognized in the MIF system and cross-reaction with other species is negligible. While IgM antibody response is an important marker for serodiagnosis of acute infection, rheumatoid factor often causes false-positive reactions. Persistent TWAR IgG antibody has been useful for seroepidemiologic studies and an association of TWAR IgG antibody and atherosclerotic diseases has been observed. IgA antibody may not be a useful marker for chronic TWAR infection or for acute infection.
本文简要介绍了目前用于衣原体微量免疫荧光（MIF）检测的方法。迄今为止，使用肺炎衣原体（TWAR）抗原的MIF血清学检测为诊断急性TWAR感染提供了最敏感和特异的方法。在原发性感染中，与沙眼衣原体感染相比，TWAR IgM抗体反应持续时间更长，IgG抗体产生较慢。与其他衣原体种类不同，在MIF系统中仅识别出肺炎衣原体的一个血清型，与其他种类的交叉反应可忽略不计。虽然IgM抗体反应是急性感染血清诊断的重要标志物，但类风湿因子常导致假阳性反应。持续存在的TWAR IgG抗体已用于血清流行病学研究，并且已观察到TWAR IgG抗体与动脉粥样硬化疾病之间的关联。IgA抗体可能不是慢性TWAR感染或急性感染的有用标志物。
