Bösing-Schneider R, Haug M
J Exp Med. 1976 Dec 1;144(6):1458-64. doi: 10.1084/jem.144.6.1458.
Nude mice were injected with antigen and T cells at different times to induce unresponsiveness to SRBC. Spleen cells derived from these mice were tested in vitro for the capability to produce antibody-forming cells against sheep erythrocytes in the presence of a T-cell-replacing factor. It was found that priming with antigen alone did not result in paralysis but a later injection of thymus-derived lymphocytes together with antigen results in unresponsiveness of these cells in vitro, provided there was an interval of several days between the in vivo administration of thymus lymphocytes and the explantations of cells to in vitro cultures.
将裸鼠在不同时间注射抗原和T细胞，以诱导对绵羊红细胞（SRBC）无反应性。从这些小鼠中获取脾细胞，在体外检测其在T细胞替代因子存在的情况下产生抗绵羊红细胞抗体形成细胞的能力。结果发现，单独用抗原致敏不会导致麻痹，但如果在体内给予胸腺来源淋巴细胞与细胞接种到体外培养之间间隔数天，那么随后将胸腺来源淋巴细胞与抗原一起注射会导致这些细胞在体外无反应性。
