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通过实时定量聚合酶链反应检测人巨细胞病毒DNA

Detection of human cytomegalovirus DNA by real-time quantitative PCR.

作者信息

Nitsche A, Steuer N, Schmidt C A, Landt O, Ellerbrok H, Pauli G, Siegert W

机构信息

Klinik für Innere Medizin m.S. Hämatologie und Onkologie, Charité-Campus Virchow Klinikum, Humboldt Universität zu Berlin, Berlin, Germany.

出版信息

J Clin Microbiol. 2000 Jul;38(7):2734-7. doi: 10.1128/JCM.38.7.2734-2737.2000.

DOI:10.1128/JCM.38.7.2734-2737.2000
PMID:10878073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC87012/
Abstract

A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.

摘要

开发了一种实时聚合酶链反应（PCR）检测方法来定量检测人巨细胞病毒（CMV）DNA。该检测方法用于证明骨髓移植患者血浆中的CMV DNA载量高于献血者血浆中的CMV DNA载量。CMV抗原阳性患者的CMV载量高于抗原阴性患者。

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本文引用的文献

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Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA.比较不同实时PCR方法用于人巨细胞病毒DNA的定量检测
Clin Chem. 1999 Nov;45(11):1932-7.
2
Quantitative polymerase chain reaction to predict occurrence of symptomatic cytomegalovirus infection and assess response to ganciclovir therapy in renal transplant recipients.定量聚合酶链反应预测肾移植受者症状性巨细胞病毒感染的发生并评估其对更昔洛韦治疗的反应
J Infect Dis. 1998 Sep;178(3):626-35. doi: 10.1086/515383.
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Evaluation of the AMPLICOR cytomegalovirus test with specimens from human immunodeficiency virus-infected subjects.使用来自人类免疫缺陷病毒感染受试者的标本对AMPLICOR巨细胞病毒检测进行评估。
J Clin Microbiol. 1998 Sep;36(9):2509-13. doi: 10.1128/JCM.36.9.2509-2513.1998.
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Diagnosis of cytomegalovirus infection in kidney transplant recipients by a quantitative RNA-DNA hybrid capture assay for cytomegalovirus DNA in leukocytes.通过定量RNA-DNA杂交捕获法检测白细胞中巨细胞病毒DNA来诊断肾移植受者的巨细胞病毒感染。
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Cytomegalovirus DNA can be detected in peripheral blood mononuclear cells from all seropositive and most seronegative healthy blood donors over time.随着时间推移，在所有血清学阳性以及大多数血清学阴性的健康献血者的外周血单个核细胞中均可检测到巨细胞病毒DNA。
Transfusion. 1998 Mar;38(3):271-8. doi: 10.1046/j.1537-2995.1998.38398222871.x.
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Longitudinal fluctuations in cytomegalovirus load in bone marrow transplant patients: relationship between peak virus load, donor/recipient serostatus, acute GVHD and CMV disease.骨髓移植患者巨细胞病毒载量的纵向波动：病毒载量峰值、供体/受体血清学状态、急性移植物抗宿主病与巨细胞病毒疾病之间的关系
Bone Marrow Transplant. 1998 Mar;21(6):597-605. doi: 10.1038/sj.bmt.1701139.
7
Demonstration of cytomegalovirus after bone marrow transplantation by polymerase chain reaction, virus culture and antigen detection in buffy coat leukocytes.通过聚合酶链反应、病毒培养以及检测血沉棕黄层白细胞中的抗原，来证实骨髓移植后巨细胞病毒的存在。
Bone Marrow Transplant. 1994 Jan;13(1):71-5.
8
Comparison of polymerase chain reaction from plasma and buffy coat with antigen detection and occurrence of immunoglobulin M for the demonstration of cytomegalovirus infection after liver transplantation.肝移植后巨细胞病毒感染的血浆和血沉棕黄层聚合酶链反应与抗原检测及免疫球蛋白M出现情况的比较
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Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leucocytes.通过血液白细胞的单克隆抗体染色对活动性巨细胞病毒感染进行快速免疫诊断。
J Med Virol. 1988 Jun;25(2):179-88. doi: 10.1002/jmv.1890250208.