通过实时定量聚合酶链反应检测人巨细胞病毒DNA
Detection of human cytomegalovirus DNA by real-time quantitative PCR.
作者信息
Nitsche A, Steuer N, Schmidt C A, Landt O, Ellerbrok H, Pauli G, Siegert W
机构信息
Klinik für Innere Medizin m.S. Hämatologie und Onkologie, Charité-Campus Virchow Klinikum, Humboldt Universität zu Berlin, Berlin, Germany.
出版信息
J Clin Microbiol. 2000 Jul;38(7):2734-7. doi: 10.1128/JCM.38.7.2734-2737.2000.
Abstract
A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.
摘要
开发了一种实时聚合酶链反应（PCR）检测方法来定量检测人巨细胞病毒（CMV）DNA。该检测方法用于证明骨髓移植患者血浆中的CMV DNA载量高于献血者血浆中的CMV DNA载量。CMV抗原阳性患者的CMV载量高于抗原阴性患者。
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