Flavell J R, Baumforth K R, Williams D M, Lukesova M, Madarova J, Noskova V, Prochazkova J, Lowe D, Kolar Z, Murray P G, Nelson P N
Biomedical Science Research Laboratories, School of Health Sciences, University of Wolverhampton, UK.
Mol Pathol. 2000 Jun;53(3):145-9. doi: 10.1136/mp.53.3.145.
In vitro the Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP-1) has been shown to upregulate expression of matrix metalloproteinase 9 (MMP-9), a member of a family of zinc dependent endopeptidases that is believed to facilitate tumour invasion and metastasis by degradation of the extracellular matrix.
To test whether the expression of MMP-9 in Hodgkin's disease correlates with EBV status and survival and to investigate whether LMP-1 expression affects MMP-9 concentrations in the Hodgkin's disease cell line, L428.
MMP-9 expression was measured by means of immunohistochemistry in a series of Hodgkin's disease tumours and this expression was correlated with EBV status and survival. The influence of LMP-1 on MMP-9 expression was also investigated in the Hodgkin's disease cell line, L428.
MMP-9 expression was demonstrated in the malignant Hodgkin and Reed-Sternberg cells of all (n = 86) formalin fixed, paraffin wax embedded Hodgkin's disease tumours examined. Although the intensity of MMP-9 immunostaining varied between cases, there was no correlation between MMP-9 expression and EBV status or survival. MMP-9 expression was also detected in a variety of non-malignant cells, including fibroblasts. MMP-9 was detected by zymography in the L428 and KMH2 Hodgkin's disease cell lines, whereas low or undetectable amounts of MMP-9 were found in the L591 Hodgkin's disease cell line. Induction of LMP-1 expression in the Hodgkin's disease cell line L428 did not result in a detectable increase in the values of MMP-9 as measured by zymography.
These results demonstrate that MMP-9 is consistently expressed by the Hodgkin and Reed-Sternberg cells of Hodgkin's disease tumours and by the Hodgkin's disease cell lines, L428 and KMH2. However, this expression does not appear to be related either to LMP-1 values or to survival.
在体外实验中，爱泼斯坦-巴尔病毒（EBV）编码的潜伏膜蛋白1（LMP-1）已被证明可上调基质金属蛋白酶9（MMP-9）的表达，MMP-9是锌依赖性内肽酶家族的一员，据信它通过降解细胞外基质促进肿瘤侵袭和转移。
检测霍奇金淋巴瘤中MMP-9的表达是否与EBV状态及生存率相关，并研究LMP-1表达是否影响霍奇金淋巴瘤细胞系L428中MMP-9的浓度。
通过免疫组织化学方法检测一系列霍奇金淋巴瘤肿瘤中MMP-9的表达，并将其与EBV状态及生存率进行关联分析。同时也在霍奇金淋巴瘤细胞系L428中研究LMP-1对MMP-9表达的影响。
在所检测的所有（n = 86）福尔马林固定、石蜡包埋的霍奇金淋巴瘤肿瘤的恶性霍奇金和里德-斯腾伯格细胞中均证实有MMP-9表达。尽管不同病例间MMP-9免疫染色强度有所不同，但MMP-9表达与EBV状态或生存率之间无相关性。在包括成纤维细胞在内的多种非恶性细胞中也检测到了MMP-9表达。通过酶谱法在L428和KMH2霍奇金淋巴瘤细胞系中检测到了MMP-9，而在L591霍奇金淋巴瘤细胞系中发现MMP-9含量较低或无法检测到。在霍奇金淋巴瘤细胞系L428中诱导LMP-1表达，通过酶谱法检测未发现MMP-9值有可检测到的增加。
这些结果表明，MMP-9在霍奇金淋巴瘤肿瘤的霍奇金和里德-斯腾伯格细胞以及霍奇金淋巴瘤细胞系L428和KMH2中持续表达。然而，这种表达似乎与LMP-1值或生存率均无关。
