Fimmel P J
J Immunol. 1975 Jul;115(1):135-8.
The role of E rosette-forming cells in leukocyte migration inhibition was investigated with human buffy coat cells fractionated by combined E rosette formation and Ficoll-Hypaque (FH) density gradient centrifugation. The migration of unfractionated dextran-sedimented leukocytes and FH-separated mononuclear cells (MN) were equally inhibited in the presence of Escherichia coli somatic antigen (E. coli), purified protein derivative of tuberculin (PPD) and influenza virus antigen (Flu). Polymorphonuclear leukocytes (PMN) separated from whole leukocytes by one FH density gradient fractionation contained greater than 1.0% E rosette-forming cells and were inhibited by E. coli and PPD. PMN submitted to three transits of FH (DF III) contained less than or equal to 0.02% E rosette forming cells and were unaffected by each of the three antigens. The migration of mixtures of 10% MN and 90DF III cells were inhibited in the presence of specific antigen, while MN depleted of E rosette-forming cells (less than or equal to 0.5%) pooled with equal numbers of DF III cells did not respond to any antigen by migration inhibition. These results show that PMN purified by three transits of FH are unable to respond to specific antigen by inhibition of migration from microcapillary tubes, and specific antigen-induced inhibition of migration of peripheral blood leukocytes does not occur in the absence of D rosette-forming cells in normal subjects.
采用通过E花环形成和Ficoll-Hypaque（FH）密度梯度离心分离的人血沉棕黄层细胞，研究了E花环形成细胞在白细胞迁移抑制中的作用。在存在大肠杆菌菌体抗原（大肠杆菌）、结核菌素纯蛋白衍生物（PPD）和流感病毒抗原（流感）的情况下，未分级的葡聚糖沉淀白细胞和FH分离的单核细胞（MN）的迁移受到同等程度的抑制。通过一次FH密度梯度分级从全白细胞中分离出的多形核白细胞（PMN）含有大于1.0%的E花环形成细胞，并受到大肠杆菌和PPD的抑制。经过三次FH处理（DF III）的PMN含有小于或等于0.02%的E花环形成细胞，并且不受这三种抗原中任何一种的影响。在存在特异性抗原的情况下，10% MN和90% DF III细胞混合物的迁移受到抑制，而与等量DF III细胞混合的缺乏E花环形成细胞（小于或等于0.5%）的MN对任何抗原均无迁移抑制反应。这些结果表明，经过三次FH处理纯化的PMN无法通过抑制从微毛细管的迁移来对特异性抗原作出反应，并且在正常受试者中，在没有D花环形成细胞的情况下，不会发生特异性抗原诱导的外周血白细胞迁移抑制。
