Hu K, Kobayashi H, Dong A, Iwasaki S, Yao X
Planta Med. 2000 Aug;66(6):564-7. doi: 10.1055/s-2000-8601.
With guidance of Pyricularia oryzae bioassay, daphnoretin (1), (+)-nortrachelogenin (2), genkwanol A (3), wikstrol A (4), wikstrol B (5) and daphnodorin B (6) were isolated from the roots of Wikstroemia indica. Compounds 1-6 induced morphological deformation of P. oryzae mycelia with MMDC values of 68.4 +/- 1.3, 31.3 +/- 1.8, 45.8 +/- 0.5, 70.1 +/- 2.4, 52.3 +/- 0.9 and 73.7 +/- 1.6 microM, respectively. Compounds 3-6 showed moderate activity against microtubule polymerization with IC50 values of 112 +/- 4, 131 +/- 3, 184 +/- 6 and 142 +/- 2 microM in vitro, respectively. Compounds 2, 3, 5 and 6 were moderately active against HIV-1 in vitro. The findings of bioactivity of 1-6 support the antifungus, antimitosis and anti-HIV-1 uses for W. indica roots.
在稻瘟病菌生物测定的指导下，从了哥王的根部分离得到了瑞香素（1）、（+）-去甲rachelogenin（2）、芫花醇A（3）、狼毒素A（4）、狼毒素B（5）和了哥王素B（6）。化合物1-6诱导稻瘟病菌丝体形态变形，其最小抑菌浓度（MMDC）值分别为68.4±1.3、31.3±1.8、45.8±0.5、70.1±2.4、52.3±0.9和73.7±1.6 microM。化合物3-6在体外对微管聚合表现出中等活性，其半数抑制浓度（IC50）值分别为112±4、131±3、184±6和142±2 microM。化合物2、3、5和6在体外对HIV-1具有中等活性。化合物1-6的生物活性研究结果支持了哥王根的抗真菌、抗有丝分裂和抗HIV-1的用途。
