Huang J, Ogihara Y, Zhang H, Shimizu N, Takeda T
Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Japan.
Phytochemistry. 2000 Aug;54(8):817-22. doi: 10.1016/s0031-9422(00)00173-4.
Two saponins were isolated from the roots of Ardisia mamillata HANCE. Their structures were established on the basis of MALDI-TOFMS, 1H, 13C NMR and 2D NMR (COSY, HOHAHA, HETCOR, HMBC and ROESY) spectra, and on chemical evidence, to be ardisimamilloside A, 3-O-[alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranosyl-(1 --> 4)-[beta-D-glucopyranosyl-(1 --> 2)]-alpha-L-arabinopyranosyl]-3beta, 16alpha,28alpha-trihydroxy-13beta,28-epoxy-oleanan+ ++-30-al; and ardisimamilloside B, 3-O-[alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranosyl-(1 --> 4)-[beta-D-glucopyranosyl-( 1 --> 2)]-alpha-L-arabinopyranosyl]-beta3-hydroxy-13beta,28- epoxy-oleanan-16-oxo-30-al.
从九节龙（Ardisia mamillata HANCE）根部分离得到两种皂苷。基于基质辅助激光解吸电离飞行时间质谱（MALDI-TOFMS）、1H、13C核磁共振（NMR）以及二维核磁共振（2D NMR，包括COSY、HOHAHA、HETCOR、HMBC和ROESY）光谱，并结合化学证据，确定它们的结构分别为九节龙皂苷A，即3-O-[α-L-鼠李糖基-(1→2)-β-D-葡萄糖基-(1→4)-[β-D-葡萄糖基-(1→2)]-α-L-阿拉伯糖基]-3β,16α,28α-三羟基-13β,28-环氧-齐墩果烷-30-醛；以及九节龙皂苷B，即3-O-[α-L-鼠李糖基-(1→2)-β-D-葡萄糖基-(1→4)-[β-D-葡萄糖基-(1→2)]-α-L-阿拉伯糖基]-β3-羟基-13β,28-环氧-齐墩果烷-16-氧代-30-醛。
