Sand L, Jalouli J, Larsson P A, Magnusson B, Hirsch J M
Department of Oral and Maxillofacial Surgery, Faculty of Odontology, Göteborg University, Sweden.
J Oral Maxillofac Surg. 2000 Oct;58(10):1129-34; discussion 1135-6. doi: 10.1053/joms.2000.9573.
This study investigated the possibility that human papilloma virus (HPV) is a possible etiologic agent in the development of ameloblastoma.
DNA was extracted from 18 formalin-fixed, paraffin-embedded biopsy specimens and assayed for the presence of HPV DNA by PCR using the L1 consensus primer and specific primers for HPV types 6/11, 16 and 18.
Eight samples (67%) were positive for HPV. Of the 8 HPV-positive samples, 7 were positive for HPV 18. Four of the HPV 18-positive samples were also positive for HPV 6/11. One HPV-positive sample was not positive for any of the type-specific primers.
No conclusions can be drawn about the etiologic role of HPV from this study, but surgical manipulation is suggested to be one of the reasons for HPV presence attributable to contamination from the surface mucosal epithelium in these tumors.
本研究调查了人乳头瘤病毒（HPV）作为成釉细胞瘤发病潜在病因的可能性。
从18份福尔马林固定、石蜡包埋的活检标本中提取DNA，使用L1共有引物和针对HPV 6/11型、16型和18型的特异性引物，通过聚合酶链反应（PCR）检测HPV DNA的存在情况。
8份样本（67%）HPV检测呈阳性。在这8份HPV阳性样本中，7份为HPV 18阳性。4份HPV 18阳性样本同时也为HPV 6/11阳性。1份HPV阳性样本对任何型特异性引物均呈阴性。
本研究无法就HPV的病因学作用得出结论，但提示手术操作可能是这些肿瘤中HPV存在的原因之一，这归因于表面黏膜上皮的污染。
