Lawther R P, Phillips S L, Cooper T G
Mol Gen Genet. 1975;137(2):89-99. doi: 10.1007/BF00341675.
The RNA polymerase inhibitor, lomofungin has been used to determine the half life of specific synthetic capacities (invertase and alpha-glucosidase) as well as that for gross protein synthesis. In both cases the studies conclude that cognate messenger RNAs decay with a half life of approximately 20 minutes. This antibiotic has been used to determine the half life of allophanate hydrolase specific synthetic capacity. We find that it decays with a half life of about three minutes; a value that agrees with the decay rates of allophanate hydrolase synthetic capacity following removal of inducer. These observations argue that mRNA may be metabolized by two separate routes in Saccharomyces.
RNA聚合酶抑制剂洛莫真菌素已被用于测定特定合成能力（转化酶和α-葡萄糖苷酶）以及总蛋白质合成的半衰期。在这两种情况下，研究得出结论，同源信使核糖核酸（mRNA）以约20分钟的半衰期衰变。这种抗生素已被用于测定脲基甲酸酯水解酶特定合成能力的半衰期。我们发现它以约三分钟的半衰期衰变；这一数值与去除诱导剂后脲基甲酸酯水解酶合成能力的衰变率一致。这些观察结果表明，在酿酒酵母中，mRNA可能通过两条独立的途径进行代谢。
