Lawther R P, Cooper T G
J Bacteriol. 1975 Mar;121(3):1064-73. doi: 10.1128/jb.121.3.1064-1073.1975.
Our previous work has shown that both induction, after addition of inducer, and loss of ability to produce allophanate hydrolase, after removal of inducer, proceed more rapidly than expected from the reported half-life of messenger ribonucleic acid in Saccharomyces cerevisiae. As a basis of rectifying these observations, we have characterized induction and repression of allophanate hydrolase synthesis and find that: (i) induction of the hydrolase begins immediately upon addition of inducer, (ii) once induction has been initiated removal of inducer does not result in immediate loss of synthetic capacity, (iii) induction of the capacity to produce hydrolase can occur in the absence of protein synthesis, (iv) the half-life of hydrolase synthetic capacity increases if protein synthesis is inhibited, (v) allophanate hydrolase itself is not degraded upon removal of inducer, and (vi) induction and repression of allophanate hydrolase synthetic capacity likely occurs at the level of transcription.
我们之前的研究表明，添加诱导剂后的诱导过程以及去除诱导剂后产生脲基甲酸酯水解酶能力的丧失，其进行速度比根据酿酒酵母中信使核糖核酸报道的半衰期所预期的要快。作为纠正这些观察结果的基础，我们对脲基甲酸酯水解酶合成的诱导和阻遏进行了表征，发现：（i）添加诱导剂后，水解酶的诱导立即开始；（ii）一旦诱导开始，去除诱导剂不会导致合成能力立即丧失；（iii）在没有蛋白质合成的情况下，也可发生产生水解酶能力的诱导；（iv）如果蛋白质合成受到抑制，水解酶合成能力的半衰期会增加；（v）去除诱导剂后，脲基甲酸酯水解酶本身不会被降解；（vi）脲基甲酸酯水解酶合成能力的诱导和阻遏可能发生在转录水平。
