Starkie R L, Angus D J, Rolland J, Hargreaves M, Febbraio M A
Exercise Physiology and Metabolism Laboratory, Department of Physiology, The University of Melbourne, Parkville 3052, Victoria, Australia.
J Physiol. 2000 Nov 1;528(Pt 3):647-55. doi: 10.1111/j.1469-7793.2000.t01-1-00647.x.
The present study was undertaken to examine the effect of exercise and carbohydrate (CHO) ingestion on intracellular monocyte cytokine production. Subjects performed 2 h of cycling at 70 % peak pulmonary O2 uptake (VO2,peak) while ingesting either an 8 % CHO beverage or a sweet placebo. Whole blood was incubated with (stimulated) or without (spontaneous) lipopolysaccharide (LPS) and surface stained for monocyte surface antigens. The cells were permeabilised, stained for intracellular cytokines and analysed using flow cytometry. Exercise had no effect on the number of monocytes spontaneously producing cytokines, but the number of stimulated IL-1alpha-, TNF-alpha- and IL-6-positive monocytes were elevated (P < 0.01) immediately post-exercise and 2 h post-exercise. These stimulated cells produced less (P < 0.05) TNF-alpha immediately post-exercise, and less (P < 0.05) TNF-alpha and IL-1alpha 2 h post-exercise. There was a small, but significant increase (P < 0.05) in the plasma IL-6 concentration immediately post-exercise. Exercise resulted in an elevation (P < 0.01) in the plasma adrenaline concentration in the placebo trial, and ingestion of CHO attenuated this increase. CHO ingestion had no effect on monocyte cytokine production, plasma IL-6 or circulating leukocyte numbers. These data suggest that circulating monocytes are not the origin of increased levels of plasma IL-6 during exercise: prolonged cycling exercise increased the number of monocytes producing cytokines upon stimulation, but these cells produced less cytokines post-exercise. In addition, attenuation of plasma adrenaline levels had no effect on plasma IL-6 or monocyte cytokine production.
本研究旨在探讨运动和碳水化合物（CHO）摄入对细胞内单核细胞细胞因子产生的影响。受试者在摄入8% CHO饮料或甜味安慰剂的同时，以70%的峰值肺氧摄取量（VO2峰值）进行2小时的骑行运动。全血在有（刺激）或无（自发）脂多糖（LPS）的情况下孵育，并对单核细胞表面抗原进行表面染色。细胞经通透处理后，对细胞内细胞因子进行染色，并使用流式细胞术进行分析。运动对自发产生细胞因子的单核细胞数量没有影响，但运动后即刻和运动后2小时，刺激后IL-1α、TNF-α和IL-6阳性单核细胞的数量增加（P<0.01）。这些受刺激的细胞在运动后即刻产生的TNF-α较少（P<0.05），在运动后2小时产生的TNF-α和IL-1α较少（P<0.05）。运动后即刻血浆IL-6浓度有小幅但显著的升高（P<0.05）。在安慰剂试验中，运动导致血浆肾上腺素浓度升高（P<0.01），而摄入CHO可减弱这种升高。摄入CHO对单核细胞细胞因子产生、血浆IL-6或循环白细胞数量没有影响。这些数据表明，循环单核细胞不是运动期间血浆IL-6水平升高的来源：长时间的骑行运动增加了受刺激时产生细胞因子的单核细胞数量，但这些细胞在运动后产生的细胞因子较少。此外，血浆肾上腺素水平的减弱对血浆IL-6或单核细胞细胞因子产生没有影响。
